Current pharmaceutical biotechnology · 2026
Pisum sativum L. Peel Extract Based Biogenic Silver Nanoparticles as Antibiotic Adjuvants to Reverse Multidrug Resistance in Bacteria.
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Batool Salma, Shahid Izzah, Iftikhar Moaiza, Haider Burhan, Deeba Farah, Saleem Rahman Shah Zaib
Аннотация
INTRODUCTION: Rapid spread of antimicrobial drug resistance is alarming and demands a sustainable solution. Green synthesis of silver nanoparticles can pave the way for the development of adjuvants that can help overcome bacterial drug resistance.
METHODS: In the current study, green synthesis of silver nanoparticles utilizing pea (Pisum sativum) peels and their evaluation against multidrug-resistant (MDR) bacterial pathogens has been proposed. Pea peels were extracted and analyzed for antioxidant potential by non-enzymatic DPPH assay. The extract was used to prepare silver nanoparticles (gAgNPs) characterized using UV-VIS spectroscopy, SEM, FTIR, and zeta sizer, and evaluated for antibacterial activity.
RESULTS: The SEM showed that the gAgNPs were spherical with a size of 59.24 nm ± 0.3, and the zeta sizer gave a PDI of 0.2. As adjuvants, these gAgNPs in combination with vancomycin showed a superior antibacterial activity against vancomycin-resistant E. faecalis (zone of inhibition= 66 mm ± 0.41) and vancomycin-resistant P. aeruginosa (35 mm ± 1.41) as compared to vancomycin. Hemolysis was IC50= 998.44 μg/mL, creating a therapeutic window for IC50 =5 μg/mL, effective against human pathogens.
CONCLUSION: This study suggests these gAgNPs could be a good lead to work as adjuvants with vancomycin against vancomycin-resistant Gram-positive and Gram-negative clinical bacteria and thus warrants further studies.
METHODS: In the current study, green synthesis of silver nanoparticles utilizing pea (Pisum sativum) peels and their evaluation against multidrug-resistant (MDR) bacterial pathogens has been proposed. Pea peels were extracted and analyzed for antioxidant potential by non-enzymatic DPPH assay. The extract was used to prepare silver nanoparticles (gAgNPs) characterized using UV-VIS spectroscopy, SEM, FTIR, and zeta sizer, and evaluated for antibacterial activity.
RESULTS: The SEM showed that the gAgNPs were spherical with a size of 59.24 nm ± 0.3, and the zeta sizer gave a PDI of 0.2. As adjuvants, these gAgNPs in combination with vancomycin showed a superior antibacterial activity against vancomycin-resistant E. faecalis (zone of inhibition= 66 mm ± 0.41) and vancomycin-resistant P. aeruginosa (35 mm ± 1.41) as compared to vancomycin. Hemolysis was IC50= 998.44 μg/mL, creating a therapeutic window for IC50 =5 μg/mL, effective against human pathogens.
CONCLUSION: This study suggests these gAgNPs could be a good lead to work as adjuvants with vancomycin against vancomycin-resistant Gram-positive and Gram-negative clinical bacteria and thus warrants further studies.