Downregulation of 14-3-3β and 14-3-3ζ in lesions of psoriasis vulgaris.

BACKGROUND

The 14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells, which play essential roles in a wide range of vital regulatory processes, including differentiation, proliferation and transformation. In mammalian cells, seven 14-3-3 isoforms (β, γ, ε, η, θ/τ, σ and ζ) have been identified, and each of these seems to have distinct tissue localizations and isoform-specific functions. 14-3-3β and 14-3-3ζ are two important members of the 14-3-3 family.

AIM: To explore the role of 14-3-3β and 14-3-3ζ in normal skin and psoriasis vulgaris (PV) skin.

METHODS

Using immunohistochemistry and western blotting, we measured expression of 14-3-3β and 14-3-3ζ in 30 PV lesions and 15 normal skin samples. The average optical density (OD) of immunostaining and the relative grey scale of immunoblotting for 4-3-3β and 14-3-3ζ were analysed by the t-test.

RESULTS

The average OD of immunostaining for 14-3-3β and 14-3-3ζ was 0.17 ± 0.00 and 0.24 ± 0.01, respectively, in psoriatic lesions, which was significantly lower than in normal controls (0.22 ± 0.01 and 0.37 ± 0.02, respectively; P < 0.01 for both). There was also a significant difference in the relative grey scale of 14-3-3β and 14-3-3ζ (0.52 ± 0.03 and 1.44 ± 0.06, respectively) in psoriatic lesions compared with normal control tissue (3.32 ± 0.15 and 2.76 ± 0.11, respectively; P < 0.01 for both).

CONCLUSIONS

Expression of 14-3-3β and 14-3-3ζ were lower in psoriatic lesions than in normal human skin tissue. We speculate that 14-3-3β and 14-3-3ζ may be involved in the regulation of normal skin function, thus decreased expression of 14-3-3β and 14-3-3ζ might precipitate the disturbance in proliferation and differentiation of keratinocytes seen in psoriasis.