[Application of dot immunogold filtration assay for detecting serum antibodies in clonorchiasis patients].

OBJECTIVE

To establish a new, fast, sensitive and specific assay for detecting antibodies in clonorchiasis.

METHODS

Using Clonorchis sinensis adult worm antigen and SPA labelled with colloidal gold as color developing agent, a dot immunogold filtration assay (DIGFA) for detecting clonorchiasis antibodies was tested. Dot-ELISA was used as the parallel control.

RESULTS

The positive rate of DIGFA and dot-ELISA in testing the sera from 119 proved cases were 96.4% (115/119) and 92.4% (110/119), respectively, the difference between the two assays being in significant (P > 0.05). The negative rate of DIGFA in healthy people was 100% (40/40). The cross reaction rate in 20 cysticercosis cases and 25 schistosomiasis cases were 5% (1/20) and 4% (1/25), respectively. Both coincidence rates comparing DIGFA with dot-ELISA were 90.9% (50/55).

CONCLUSION

DIGFA is as sensitive and specific as the dot-ELISA, and has the advantages of simplicity and without specific equipment.