BMC infectious diseases · 2026
Whole genome sequencing of SARS-CoV-2: evaluating the genetic diversity of variants circulating in Ebonyi State, Southeastern Nigeria.
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Odeh Elizabeth Chibuzo, Iroha Chidinma Stacy, Dowe Ejiro, Moses Ikechukwu Benjamin, Ahmed Muhammad Iluoreh, Eromon Philomena, Nwojiji Eric Chinonso, Ojide Chiedozie Kingsley, Achi Ezeora Christian, Iroha Ifeanyichukwu Romanus
Аннотация
BACKGROUND: COVID-19 is primarily considered a viral respiratory and vascular illness as its causative agent, SARS-CoV-2, predominantly targets the respiratory and vascular systems. Since being declared a global pandemic by the WHO in 2020, SARS-CoV-2 has spread to 223 countries with more than 281 million cases, and an estimated 5.4 million deaths reported globally. The cardinal aim of this study was to assess the genetic diversity of the circulating SARS-CoV-2 in Ebonyi State, Southeastern Nigeria.
METHODS: Over a span of 24 calendar months (April, 2020 to March, 2022), a total of 20 clinical samples (nasopharyngeal, nasal, and oropharyngeal swabs) from individuals who were previously confirmed to be positive for the SAR-COV-2 virus by real-time polymerase chain reaction (qRT-PCR) were randomly selected and submitted for whole genome sequencing using illumina MiSeq sequencer.
RESULTS: Results indicated a very high predominance of SARS-CoV-2 clade 21D (Eta) and pangolin lineage B.1.525, as 19 out of the 20 sequenced strains belonged to this group, while only one belonged to clade 21B and pangolin lineage B.1.1.318.
CONCLUSIONS: This study highlighted the dynamics of the circulating SARS-CoV-2 strains in Nigeria. Information from this study will be very crucial in developing and structuring interventions, adaptation of response strategies, and improved preparedness for potential future public health challenges posed by SARS-CoV-2 strains, with a resultant impact of contributing to the broader global effort in combating the pandemic.
METHODS: Over a span of 24 calendar months (April, 2020 to March, 2022), a total of 20 clinical samples (nasopharyngeal, nasal, and oropharyngeal swabs) from individuals who were previously confirmed to be positive for the SAR-COV-2 virus by real-time polymerase chain reaction (qRT-PCR) were randomly selected and submitted for whole genome sequencing using illumina MiSeq sequencer.
RESULTS: Results indicated a very high predominance of SARS-CoV-2 clade 21D (Eta) and pangolin lineage B.1.525, as 19 out of the 20 sequenced strains belonged to this group, while only one belonged to clade 21B and pangolin lineage B.1.1.318.
CONCLUSIONS: This study highlighted the dynamics of the circulating SARS-CoV-2 strains in Nigeria. Information from this study will be very crucial in developing and structuring interventions, adaptation of response strategies, and improved preparedness for potential future public health challenges posed by SARS-CoV-2 strains, with a resultant impact of contributing to the broader global effort in combating the pandemic.