The Journal of experimental medicine · 2024
Maturation of germinal center B cells after influenza virus vaccination in humans.
СтатьяПоддержка NIH: внешняя программаПоддержка службы здравоохранения СШАНазвание и аннотация: Перевод пока не подготовлен
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McIntire Katherine M, Meng Hailong, Lin Ting-Hui, Kim Wooseob, Moore Nina E, Han Julianna, McMahon Meagan, Wang Meng, Malladi Sameer Kumar, Mohammed Bassem M, Zhou Julian Q, Schmitz Aaron J, Hoehn Kenneth B, Carreño Juan Manuel, Yellin Temima, Suessen Teresa, Middleton William D, Teefey Sharlene A, Presti Rachel M, Krammer Florian, Turner Jackson S, Ward Andrew B, Wilson Ian A, Kleinstein Steven H, Ellebedy Ali H
Аннотация
Germinal centers (GC) are microanatomical lymphoid structures where affinity-matured memory B cells and long-lived bone marrow plasma cells are primarily generated. It is unclear how the maturation of B cells within the GC impacts the breadth and durability of B cell responses to influenza vaccination in humans. We used fine needle aspiration of draining lymph nodes to longitudinally track antigen-specific GC B cell responses to seasonal influenza vaccination. Antigen-specific GC B cells persisted for at least 13 wk after vaccination in two out of seven individuals. Monoclonal antibodies (mAbs) derived from persisting GC B cell clones exhibit enhanced binding affinity and breadth to influenza hemagglutinin (HA) antigens compared with related GC clonotypes isolated earlier in the response. Structural studies of early and late GC-derived mAbs from one clonal lineage in complex with H1 and H5 HAs revealed an altered binding footprint. Our study shows that inducing sustained GC reactions after influenza vaccination in humans supports the maturation of responding B cells.