The Journal of infectious diseases · 2009
Immunization with a nontoxic mutant of staphylococcal enterotoxin A, SEAD227A, protects against enterotoxin-induced emesis in house musk shrews.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Hu Dong-Liang, Omoe Katsuhiko, Sashinami Hiroshi, Shinagawa Kunihiro, Nakane Akio
Аннотация
BACKGROUND: Staphylococcal enterotoxins (SEs) are the most common cause of foodborne diseases and toxic shock throughout the world. However, no vaccine that prevents emesis induced by SEs has been described.
METHODS: A nontoxic mutant of SEA, SEAD227A, was constructed by site-directed mutagenesis and was purified by means of the Escherichia coli expression system. House musk shrews, a small emetic animal model, were immunized with SEAD227A and then challenged with wild-type SEA. SEA-induced emesis was recorded for 3 h. Antibody production was analyzed by gel double-immunodiffusion assay. Neutralizing activities of the antibodies with respect to superantigenic and emetic activities were analyzed in vitro and in vivo.
RESULTS: SEAD227A was devoid of both superantigenic and emetic activities but still retained its immunological activity. Immunization with SEAD227A strongly induced specific antibody production and provided significant protection against SEA-induced emesis. Antibodies from immunized shrews markedly inhibited the SEA-induced proliferation of spleen cells and also significantly ablated SEA-induced vomiting in the animals.
CONCLUSIONS: These results suggest that vaccination with SEAD227A, which is devoid of toxic properties, provides protection against SEA-induced emesis. This nontoxic mutant and its specific antibodies might be useful in the prevention and treatment of staphylococcal food poisoning.
METHODS: A nontoxic mutant of SEA, SEAD227A, was constructed by site-directed mutagenesis and was purified by means of the Escherichia coli expression system. House musk shrews, a small emetic animal model, were immunized with SEAD227A and then challenged with wild-type SEA. SEA-induced emesis was recorded for 3 h. Antibody production was analyzed by gel double-immunodiffusion assay. Neutralizing activities of the antibodies with respect to superantigenic and emetic activities were analyzed in vitro and in vivo.
RESULTS: SEAD227A was devoid of both superantigenic and emetic activities but still retained its immunological activity. Immunization with SEAD227A strongly induced specific antibody production and provided significant protection against SEA-induced emesis. Antibodies from immunized shrews markedly inhibited the SEA-induced proliferation of spleen cells and also significantly ablated SEA-induced vomiting in the animals.
CONCLUSIONS: These results suggest that vaccination with SEAD227A, which is devoid of toxic properties, provides protection against SEA-induced emesis. This nontoxic mutant and its specific antibodies might be useful in the prevention and treatment of staphylococcal food poisoning.