Ophthalmic research · 2020
Higher TGF-β1, TGF-β2, MMP-2, and TIMP-1 Levels in the Aqueous Humor of Patients with Acute Primary Angle Closure.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Chen Ying, Yan Hong, Li Guo, Zhang Yu
Аннотация
PURPOSE: To assess the quantitative differences in the levels of members of the transforming growth factor (TGF-β), matrix metalloproteinase (MMP), and tissue inhibitor of MMP (TIMP) families in the aqueous humor (AH) between patients with acute primary angle closure (APAC) and those with cataract only.
METHODS: AH samples were collected from 26 patients with APAC and cataract as well as 26 patients with age-related cataract only. Multiplex assays were used to measure the concentrations of TGF-β1, TGF-β2, and TGF-β3; MMP-1, MMP-2, MMP-7, MMP-9, and MMP-10; and TIMP-1 and TIMP-2.
RESULTS: The concentrations of TGF-β1, TGF-β2, MMP-2, (p < 0.001), and TIMP-1 were significantly higher (all p < 0.001) in AH samples from patients with APAC versus cataract only. Conversely, the AH concentrations of MMP-7 (p = 0.524), MMP-9 (p = 0.103), MMP-10 (p = 0.111), and TIMP-2 (p = 0.059) did not significantly differ between the groups. The concentrations of TGF-β3 and MMP-1 were below the respective detection limits in most AH samples.
CONCLUSION: The AH levels of TGF-β1, TGF-β2, MMP-2, and TIMP-1 were elevated in APAC eyes. Such altered protein levels could induce abnormal deposition of extracellular matrix in the trabecular meshwork, resulting in an increase in aqueous outflow resistance and, thereby, providing a possible explanation of the mechanism of residual glaucoma after cataract surgery.
METHODS: AH samples were collected from 26 patients with APAC and cataract as well as 26 patients with age-related cataract only. Multiplex assays were used to measure the concentrations of TGF-β1, TGF-β2, and TGF-β3; MMP-1, MMP-2, MMP-7, MMP-9, and MMP-10; and TIMP-1 and TIMP-2.
RESULTS: The concentrations of TGF-β1, TGF-β2, MMP-2, (p < 0.001), and TIMP-1 were significantly higher (all p < 0.001) in AH samples from patients with APAC versus cataract only. Conversely, the AH concentrations of MMP-7 (p = 0.524), MMP-9 (p = 0.103), MMP-10 (p = 0.111), and TIMP-2 (p = 0.059) did not significantly differ between the groups. The concentrations of TGF-β3 and MMP-1 were below the respective detection limits in most AH samples.
CONCLUSION: The AH levels of TGF-β1, TGF-β2, MMP-2, and TIMP-1 were elevated in APAC eyes. Such altered protein levels could induce abnormal deposition of extracellular matrix in the trabecular meshwork, resulting in an increase in aqueous outflow resistance and, thereby, providing a possible explanation of the mechanism of residual glaucoma after cataract surgery.