Zhonghua nei ke za zhi · 2000
[Distribution of interleukin-6 receptors (CD(126) and CD(w130)) on circulating lymphocytes in patients with lupus nephritis].
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Zeng L, Sun L, Ye R, Xu H, Yu X, Kong Q, Zhou F
Аннотация
OBJECTIVE: To study the distribution of IL-6 receptors (CD(126) and CD(w130)) on lymphocytes in patients with lupus nephritis (LN).
METHOD: The percentages of lymphocyte subsets were examined by direct two colour immunofluorescence on flow cytometry.
RESULTS: (1) In active and inactive cases, the percentages of CD(+)(4) cells obviously decreased as compared with the normal controls (P < 0.01). Increased numbers of CD(+)(8) cells could be detected in LN patients (P < 0.05), especially in active cases (P < 0.01). Lymphocytes of active cases contained significantly greater percentage of CD(+)(23) cells than those of normal subjects (P < 0.05). On the contrary, the percentage of CD(+)(23) cells was statistically lower in the inactive cases than in normal subjects (P < 0.01). No significant difference in the percentages of CD(+)(126) and CD(+)(w130) cells was observed among the active, inactive and healthy groups (P > 0.05); (2) The proportion of CD(+)(4) CD(+)(126) and CD(+)(4) CD(+)(w130) cells in total lymphocytes was significantly lower in the active than in the inactive ones or normal subjects (P < 0.05; P < 0.01), whereas that of CD(+)(8) CD(+)(126) and CD(+)(8) CD(+)(w130) cells was not different among the active, inactive and normal subjects (P > 0.05). The proportion of CD(+)(23) CD(+)(126) cells and CD(+)(23) CD(+)(w130) cells augmented significantly in the active (P < 0.05), whereas it lessened in the inactive without statistical meaning as compared with the normal subjects (P > 0.05). (3) There was a positive correlation between the percentage of CD(+)(23) CD(+)(126) cells and that of CD(+)(23) CD(+)(w130) cells in total lymphocytes in the active group (r = 0.756, P < 0.05). However, no correlation between the percentage of CD(+)(23) CD(+)(126) cells and that of CD(+)(23) CD(+)(w130) cells in total lymphocytes was found in the inactive (r = 0.376, P > 0.05) or normal group (r = 0.256, P > 0.05); (4) The fraction of CD(+)(23) cells expressing CD(126) and CD(w130) was related to the SLE disease activity index (SLEDAI) and lymphopenia (P < 0.05, P < 0.005). But it was neither associated with the number of damaged organs and the degree of damage, not with that of ANA, anti-dsDNA antibody and complements (C(3) and C(4)) (P > 0.05).
CONCLUSIONS: LN patients present not only B cell hyperactivity but also T cell abnormality. In active cases, there is abnormal distribution of IL-6 receptors on lymphocytes: reduced CD(126) and CD(w130) expression on CD(+)(4) cells and elevated CD(126) and CD(w130) expression on CD(+)(23) cells. Detecting the proportion of CD(+)(23) CD(+)(126) cells or CD(+)(23) CD(+)(w130) cells in total lymphocytes may serve as one of activity indexes for LN patients.
METHOD: The percentages of lymphocyte subsets were examined by direct two colour immunofluorescence on flow cytometry.
RESULTS: (1) In active and inactive cases, the percentages of CD(+)(4) cells obviously decreased as compared with the normal controls (P < 0.01). Increased numbers of CD(+)(8) cells could be detected in LN patients (P < 0.05), especially in active cases (P < 0.01). Lymphocytes of active cases contained significantly greater percentage of CD(+)(23) cells than those of normal subjects (P < 0.05). On the contrary, the percentage of CD(+)(23) cells was statistically lower in the inactive cases than in normal subjects (P < 0.01). No significant difference in the percentages of CD(+)(126) and CD(+)(w130) cells was observed among the active, inactive and healthy groups (P > 0.05); (2) The proportion of CD(+)(4) CD(+)(126) and CD(+)(4) CD(+)(w130) cells in total lymphocytes was significantly lower in the active than in the inactive ones or normal subjects (P < 0.05; P < 0.01), whereas that of CD(+)(8) CD(+)(126) and CD(+)(8) CD(+)(w130) cells was not different among the active, inactive and normal subjects (P > 0.05). The proportion of CD(+)(23) CD(+)(126) cells and CD(+)(23) CD(+)(w130) cells augmented significantly in the active (P < 0.05), whereas it lessened in the inactive without statistical meaning as compared with the normal subjects (P > 0.05). (3) There was a positive correlation between the percentage of CD(+)(23) CD(+)(126) cells and that of CD(+)(23) CD(+)(w130) cells in total lymphocytes in the active group (r = 0.756, P < 0.05). However, no correlation between the percentage of CD(+)(23) CD(+)(126) cells and that of CD(+)(23) CD(+)(w130) cells in total lymphocytes was found in the inactive (r = 0.376, P > 0.05) or normal group (r = 0.256, P > 0.05); (4) The fraction of CD(+)(23) cells expressing CD(126) and CD(w130) was related to the SLE disease activity index (SLEDAI) and lymphopenia (P < 0.05, P < 0.005). But it was neither associated with the number of damaged organs and the degree of damage, not with that of ANA, anti-dsDNA antibody and complements (C(3) and C(4)) (P > 0.05).
CONCLUSIONS: LN patients present not only B cell hyperactivity but also T cell abnormality. In active cases, there is abnormal distribution of IL-6 receptors on lymphocytes: reduced CD(126) and CD(w130) expression on CD(+)(4) cells and elevated CD(126) and CD(w130) expression on CD(+)(23) cells. Detecting the proportion of CD(+)(23) CD(+)(126) cells or CD(+)(23) CD(+)(w130) cells in total lymphocytes may serve as one of activity indexes for LN patients.