Whole transcriptome sequencing and ceRNA regulatory network in diabetic peripheral neuropathy.

UNLABELLED: Although diabetic peripheral neuropathy (DPN) is a serious complication of diabetes, its molecular causes are still unclear. The aim of this study was therefore to further investigate the pathogenesis of diabetes through whole transcriptome sequencing.

METHODS

We performed whole transcriptome sequencing on sciatic nerve samples from Sprague-Dawley (SD) rats. Based on the whole transcriptome sequencing data, the differentially expressed genes (DEGs) between the DPN and the control groups were identified, and functions and pathways in which DEGs were enriched were analyzed. Thereafter, the protein-protein interaction (PPI) network was built, and the top 20 hub genes were screened via STRING algorithm. In addition, enrichment analysis for the top 20 hub genes was subsequently performed. Finally, the competing endogenous RNA (ceRNA) network was created.

RESULTS

There were 597 DEGs between the DPN group and the control groups. The top 20 hub genes were Fos, Tnf, Jun, Cd3e, Cd79a, Cd27, Cd79b, Ptprc, Cd22, Cd40lg, Cd19, Cr2, Sell, Il6, Pdlim3, Actn2, Myom2, Tnni2, Ldb3, and Myh7. According to the enrichment analysis, DEGs and hub genes were enriched into the following: 'B cell receptor signaling pathway', 'TNF signaling pathway', 'T cell receptor signaling pathway' 'Th17 cell differentiation', 'IL-17 signaling pathway', 'primary immunodeficiency', and 'toll-like receptor signaling pathway'. The ceRNA network finally contained 9 mRNAs, 23 miRNAs, and 23 lncRNAs.

CONCLUSIONS

This study was able to determine that hub genes such as Fos and Jun may be involved in the progression of DPN by regulating signaling pathways like the B cell receptor signaling pathway, the TNF signaling pathway, and the IL-17 signaling pathway. The findings of this study could provide novel insights into the roles of DEGs in the pathogenesis of DPN.