Molecular therapy. Methods & clinical development · 2020
CRISPR-Cas9 gene editing of hepatitis B virus in chronically infected humanized mice.
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Stone Daniel, Long Kelly R, Loprieno Michelle A, De Silva Feelixge Harshana S, Kenkel Elizabeth J, Liley R Matt, Rapp Stephen, Roychoudhury Pavitra, Nguyen Thuy, Stensland Laurence, Colón-Thillet Rossana, Klouser Lindsay M, Weber Nicholas D, Le Connie, Wagoner Jessica, Goecker Erin A, Li Alvason Zhenhua, Eichholz Karsten, Corey Lawrence, Tyrrell D Lorne, Greninger Alexander L, Huang Meei-Li, Polyak Stephen J, Aubert Martine, Sagartz John E, Jerome Keith R
Аннотация
Chronic hepatitis B virus (HBV) infection is a major public health problem. New treatment approaches are needed because current treatments do not target covalently closed circular DNA (cccDNA), the template for HBV replication, and rarely clear the virus. We harnessed adeno-associated virus (AAV) vectors and CRISPR-Staphylococcus aureus (Sa)Cas9 to edit the HBV genome in liver-humanized FRG mice chronically infected with HBV and receiving entecavir. Gene editing was detected in livers of five of eight HBV-specific AAV-SaCas9-treated mice, but not control mice, and mice with detectable HBV gene editing showed higher levels of SaCas9 delivery to HBV+ human hepatocytes than those without gene editing. HBV-specific AAV-SaCas9 therapy significantly improved survival of human hepatocytes, showed a trend toward decreasing total liver HBV DNA and cccDNA, and was well tolerated. This work provides evidence for the feasibility and safety of in vivo gene editing for chronic HBV infections, and it suggests that with further optimization, this approach may offer a plausible way to treat or even cure chronic HBV infections.