Nature communications · 2021
Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Stangherlin Alessandra, Watson Joseph L, Wong David C S, Barbiero Silvia, Zeng Aiwei, Seinkmane Estere, Chew Sew Peak, Beale Andrew D, Hayter Edward A, Guna Alina, Inglis Alison J, Putker Marrit, Bartolami Eline, Matile Stefan, Lequeux Nicolas, Pons Thomas, Day Jason, van Ooijen Gerben, Voorhees Rebecca M, Bechtold David A, Derivery Emmanuel, Edgar Rachel S, Newham Peter, O'Neill John S
Аннотация
Between 6-20% of the cellular proteome is under circadian control and tunes mammalian cell function with daily environmental cycles. For cell viability, and to maintain volume within narrow limits, the daily variation in osmotic potential exerted by changes in the soluble proteome must be counterbalanced. The mechanisms and consequences of this osmotic compensation have not been investigated before. In cultured cells and in tissue we find that compensation involves electroneutral active transport of Na+, K+, and Cl- through differential activity of SLC12A family cotransporters. In cardiomyocytes ex vivo and in vivo, compensatory ion fluxes confer daily variation in electrical activity. Perturbation of soluble protein abundance has commensurate effects on ion composition and cellular function across the circadian cycle. Thus, circadian regulation of the proteome impacts ion homeostasis with substantial consequences for the physiology of electrically active cells such as cardiomyocytes.