The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation · 2005
Activation of mitogen-activated protein kinases during human lung transplantation.
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Sakiyama Shoji, Hamilton Julia, Han Bing, Jiao Yang, Shen-Tu Grace, de Perrot Marc, Keshavjee Shaf, Liu Mingyao
Аннотация
BACKGROUND: Ischemia-reperfusion is one of the unavoidable steps in lung transplantation; it is associated with acute inflammatory responses and cell death. The intracellular signal transduction mechanisms of these events are largely unknown. We hypothesize that activation of mitogen-activated protein kinases (MAPKs) is one of the important signaling events during human lung transplantation.
METHODS: Lung tissue biopsies were performed on 15 patients undergoing transplantation: after cold ischemic preservation; after warm ischemia (implantation); and after 1- or 2-hour reperfusion. The phosphorylation status of MAPK isoforms (ERK, p38-MAPK and JNK) was examined by Western blotting.
RESULTS: Phosphorylation of ERK was dramatically increased during the first 2 hours of reperfusion. Phosphorylation of JNK also significantly increased at lower levels. In contrast, phosphorylation of p38 showed no significant changes.
CONCLUSIONS: We speculate that the rapid and sustained activation of ERK and JNK during the early reperfusion period may contribute to acute inflammatory responses and cell death of lung grafts.
METHODS: Lung tissue biopsies were performed on 15 patients undergoing transplantation: after cold ischemic preservation; after warm ischemia (implantation); and after 1- or 2-hour reperfusion. The phosphorylation status of MAPK isoforms (ERK, p38-MAPK and JNK) was examined by Western blotting.
RESULTS: Phosphorylation of ERK was dramatically increased during the first 2 hours of reperfusion. Phosphorylation of JNK also significantly increased at lower levels. In contrast, phosphorylation of p38 showed no significant changes.
CONCLUSIONS: We speculate that the rapid and sustained activation of ERK and JNK during the early reperfusion period may contribute to acute inflammatory responses and cell death of lung grafts.
Предметные рубрики MeSH
AdolescentAdultBlotting, WesternCell DeathElectrophoresis, Gel, Two-DimensionalEnzyme ActivationExtracellular Signal-Regulated MAP KinasesFemaleHumansImmunoblottingInflammationLung TransplantationMAP Kinase Kinase 4MaleMiddle AgedPhosphorylationReperfusion InjurySignal Transductionp38 Mitogen-Activated Protein Kinases