[Zhonghua yan ke za zhi] Chinese journal of ophthalmology · 2000
[Cultured bovine corneal epithelial cells express functional water channel].
Аннотация на английскомСтатьяПоддержка NIH: внешняя программаПоддержка службы здравоохранения СШАНазвание и аннотация: Перевод пока не подготовлен
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Kang F, Kunyan K, Fischbarg J
Аннотация
OBJECTIVE: To investigate the water permeability (P(f)) of the cultured bovine corneal epithelial cells (CBCEC) and whether aquaporins (AQPs) were present.
METHODS: Laser light-scattering system was used to determine the volume change of CBCEC from isotonic status rapidly transferred to hypotonic one, and the P(f) value was calculated. Messenger RNA obtained from CBCEC was injected into Xenopus laevis oocytes. After four days, the P(f) was determined by monitoring volume changes of oocytes upon hypoosmotic challenge (180 mOsm/L changed to 15 mOsm/L).
RESULTS: CBCEC challenged with 10% hypoosmotic solution at 37 degrees C swelled rapidly; the cellular P(f) was 72 microm/s. In the oocytes injected with mRNA from CBCEC, the P(f) increased to 76 microm/s. This increase was inhibited (by 89%) by 0.3 mmol/L HgCl(2).
CONCLUSION: Our results suggest that CBCEC express a typical mercurial-sensitive functional water channel in good numbers, judging from the comparatively high P(f) we measured. These begin to reopen the question of the presence and extent of an epithelial role in control of corneal hydration.
METHODS: Laser light-scattering system was used to determine the volume change of CBCEC from isotonic status rapidly transferred to hypotonic one, and the P(f) value was calculated. Messenger RNA obtained from CBCEC was injected into Xenopus laevis oocytes. After four days, the P(f) was determined by monitoring volume changes of oocytes upon hypoosmotic challenge (180 mOsm/L changed to 15 mOsm/L).
RESULTS: CBCEC challenged with 10% hypoosmotic solution at 37 degrees C swelled rapidly; the cellular P(f) was 72 microm/s. In the oocytes injected with mRNA from CBCEC, the P(f) increased to 76 microm/s. This increase was inhibited (by 89%) by 0.3 mmol/L HgCl(2).
CONCLUSION: Our results suggest that CBCEC express a typical mercurial-sensitive functional water channel in good numbers, judging from the comparatively high P(f) we measured. These begin to reopen the question of the presence and extent of an epithelial role in control of corneal hydration.