Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology · 2010
Endothelial cells of oral pyogenic granulomas express eNOS and CD105/endoglin: an immunohistochemical study.
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Vassilopoulos Spyridon I, Tosios Konstantinos I, Panis Vassilios G, Vrotsos John A
Аннотация
BACKGROUND: The pyogenic granuloma (PG) is a common localized hyperplastic lesion of the oral cavity. The purpose of the present study was to investigate the immunohistochemical expression of endothelial nitric oxide synthases (eNOS) and CD105/endoglin in oral PGs, to evaluate their involvement in the angiogenetic pathways of the lesion.
MATERIALS AND METHODS: Ninety-three PGs were included in the study. Sixteen tumors were further sub-classified as pregnancy tumors (PT) and seventeen as pyogenic granulomas with fibrosis (PGFM). Immunohistochemical expression of eNOS and CD105/endoglin was quantified by computerized image analysis with a semi-automated system. Percentage of staining and number of objects (positive vessels) were recorded for each case.
RESULTS: Intense eNOS expression was seen in 92 of 93 lesions. A statistically significant association was found between eNOS percentage of staining/eNOS positive vascular spaces (objects) and age of the patients (9% increase per decade of life). Approximately 40% less eNOS positive objects were recorded in PGFM compared with PGs. Intense membranous CD105/endoglin expression was seen in all cases. The percentage of CD105/endoglin staining was statistically increased in PGs compared with PT. Approximately 40% less CD105/endoglin objects were found in PGFM compared with PGs; 56% more CD105/endoglin objects were found in tongue lesions, compared with gingival lesions. There was no statistically significant correlation considering percentage of staining and number of objects between CD105/endoglin and eNOS.
CONCLUSIONS: It is suggested that eNOS and CD105/endoglin are involved in the angiogenetic pathways of PG.
MATERIALS AND METHODS: Ninety-three PGs were included in the study. Sixteen tumors were further sub-classified as pregnancy tumors (PT) and seventeen as pyogenic granulomas with fibrosis (PGFM). Immunohistochemical expression of eNOS and CD105/endoglin was quantified by computerized image analysis with a semi-automated system. Percentage of staining and number of objects (positive vessels) were recorded for each case.
RESULTS: Intense eNOS expression was seen in 92 of 93 lesions. A statistically significant association was found between eNOS percentage of staining/eNOS positive vascular spaces (objects) and age of the patients (9% increase per decade of life). Approximately 40% less eNOS positive objects were recorded in PGFM compared with PGs. Intense membranous CD105/endoglin expression was seen in all cases. The percentage of CD105/endoglin staining was statistically increased in PGs compared with PT. Approximately 40% less CD105/endoglin objects were found in PGFM compared with PGs; 56% more CD105/endoglin objects were found in tongue lesions, compared with gingival lesions. There was no statistically significant correlation considering percentage of staining and number of objects between CD105/endoglin and eNOS.
CONCLUSIONS: It is suggested that eNOS and CD105/endoglin are involved in the angiogenetic pathways of PG.
Предметные рубрики MeSH
AdultAntigens, CDEndoglinEndothelial CellsFemaleGingival DiseasesGranuloma, PyogenicHumansImmunoenzyme TechniquesMaleMiddle AgedMouth DiseasesNeovascularization, PathologicNitric Oxide Synthase Type IIIPregnancyPregnancy ComplicationsReceptors, Cell SurfaceRetrospective StudiesTongue DiseasesYoung Adult