Effects of high-level ghrelin on intestinal epithelial cell proliferation, nutrient transport and intestinal mucosal immune barrier in chickens.

1. Chicken ghrelin (GH) plays an important role in regulating growth hormone secretion, immunity and gastrointestinal motility. This study utilised haematoxylin-eosin staining, quantitative reverse transcription PCR and western blotting to examine the effects of high-level ghrelin on the proliferation of small intestinal epithelial cells, intestinal nutrient transport and the mucosal immune barrier in chicks.2. Eighty, 17-d-old layer type chicks were randomly divided into two groups: control (C treated with sterile phosphate buffer) and the ghrelin-treated group (GH; intraperitoneally injected with 0.5 nM GH per 100 g body weight). At 1, 3 and 5 d post-injection, six chicks from each group were randomly selected for sampling of the duodenum and ileum.3. Administering GH reduced the expression of proliferating cell nuclear antigen protein in the duodenum and leucine-rich repeat-containing G protein-coupled receptor 5 mRNA in both the duodenum and ileum. In addition, GH affected villus height and ratio of villus height to crypt (H/C) depth in these sections and fatty acid binding protein 6 expression in the ileum. The relative mRNA levels of oligopeptide transporter 1, solute carrier family 3 member 1, solute carrier family 1 member 1 and solute carrier family 5 member 1 were decreased by GH.4. Birds treated with GH had a decrease in duodenal intraepithelial lymphocytes, Paneth cells and ileal goblet cells. There was a reduction in mucin 2 mRNA in goblet cells and lysozyme C and phospholipaseA2 mRNA in Paneth cells. Additionally, the relative mRNA levels of avian β-defensin 1 (AvBD1), AvBD6 and AvBD7 in the duodenum and ileum decreased with GH administration.5. The GH inhibited proliferation of chicken duodenal epithelial cells and decreased surface area available for intestinal villus absorption. This affected the transport of intestinal amino acids, glucose and bile acids and impaired the function of the mucosal immune barrier in both the duodenum and ileum.