European journal of nuclear medicine and molecular imaging · 2026
LiDAR-registered freehand SPECT combined with NIR-fluorescence enables spatial localisation of tracer-avid inguinal lymph nodes: A clinical feasibility study.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Verdijk Rick W A, Pisano Giusi, van Oosterom Matthias N, de Groot Evie, Zuurdeeg Helena, Brouwer Oscar R, van der Poel Henk G, van Leeuwen Fijs W B
Аннотация
PURPOSE: Conventional ex vivo molecular assessment of nodal specimens lacks three-dimensional (3D) spatial context, making localisation of tracer-avid sentinel nodes (SNs) within large en bloc inguinal lymph node dissection (ILND) specimens challenging. We evaluated the technical feasibility of combining light detection and ranging (LiDAR)-registered freehand SPECT (fhSPECT) with near-infrared (NIR) fluorescence imaging for spatially augmented localisation of hybrid tracer uptake in solitary SNs and SNs residing within ILND specimens.
METHODS: Fourteen patients with penile cancer (25 cN0 groins; 3 cN1 groins) underwent radio- and fluorescence-guided lymph node (LN) surgery following preoperative lymphoscintigraphy and SPECT/CT. Resected specimens underwent conventional ex vivo assessment, followed by LiDAR-registered fhSPECT with co-registered NIR-fluorescence imaging. These 3D models were compared with conventional ex vivo assessments, preoperative imaging, and histopathological outcomes.
RESULTS: In total, 40 solitary tracer-avid LNs and 3 ILND specimens underwent ex vivo assessment. LiDAR/fhSPECT localised radioactive hotspots in 38/40 solitary LNs, consistent with conventional gamma probe assessment, while both conventional and LiDAR-registered NIR-fluorescence imaging detected fluorescent signal in all 40 LNs. Among the 3 ILND procedures, preoperative SPECT/CT identified 6 tracer-avid hotspots within the anatomical resection template. Conventional gamma probe and NIR-fluorescence detected only 4 and 5 hotspots, respectively, whereas LiDAR/fhSPECT localised all 6 hotspots in the corresponding resected specimens. Histopathology identified 6 tumour-positive LNs, all of which were hybrid tracer-avid and effectively localised by LiDAR/fhSPECT.
CONCLUSION: LiDAR-registered 3D surface mapping provided anatomical context to radioactive and fluorescent molecular imaging signals, enabling localisation of tracer-avid LNs in complex ex vivo ILND specimens. These findings support the feasibility of spatially augmented localisation of hybrid tracer uptake and warrant validation in larger studies.
METHODS: Fourteen patients with penile cancer (25 cN0 groins; 3 cN1 groins) underwent radio- and fluorescence-guided lymph node (LN) surgery following preoperative lymphoscintigraphy and SPECT/CT. Resected specimens underwent conventional ex vivo assessment, followed by LiDAR-registered fhSPECT with co-registered NIR-fluorescence imaging. These 3D models were compared with conventional ex vivo assessments, preoperative imaging, and histopathological outcomes.
RESULTS: In total, 40 solitary tracer-avid LNs and 3 ILND specimens underwent ex vivo assessment. LiDAR/fhSPECT localised radioactive hotspots in 38/40 solitary LNs, consistent with conventional gamma probe assessment, while both conventional and LiDAR-registered NIR-fluorescence imaging detected fluorescent signal in all 40 LNs. Among the 3 ILND procedures, preoperative SPECT/CT identified 6 tracer-avid hotspots within the anatomical resection template. Conventional gamma probe and NIR-fluorescence detected only 4 and 5 hotspots, respectively, whereas LiDAR/fhSPECT localised all 6 hotspots in the corresponding resected specimens. Histopathology identified 6 tumour-positive LNs, all of which were hybrid tracer-avid and effectively localised by LiDAR/fhSPECT.
CONCLUSION: LiDAR-registered 3D surface mapping provided anatomical context to radioactive and fluorescent molecular imaging signals, enabling localisation of tracer-avid LNs in complex ex vivo ILND specimens. These findings support the feasibility of spatially augmented localisation of hybrid tracer uptake and warrant validation in larger studies.