Open forum infectious diseases · 2025
Relationship Between Cytokine Level and Viral Load in Severe Fever with Thrombocytopenia Syndrome.
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Guo Lili, Jiang Hao
Аннотация
BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is a viral hemorrhagic fever caused by the SFTS virus (SFTSV), a tick-borne Phlebovirus. This study investigates the association between viral load and cytokine levels in patients with SFTS.
METHODS: Viral RNA was isolated from plasma collected in EDTA and quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR). Cytokines in the sera of SFTS patients were measured using cytometric bead array (CBA).
RESULTS: Viral loads ranged from 2.3 × 103 copies/mL to 7.9 × 1011 copies/mL. Of the 46 patients, 10 (21.7%) died during treatment. Viral loads were ≥1 × 108 copies/mL in the patients who died. The concentrations of G-CSF, IFN-α, IFN-γ, IL-1β, IL-6, IL-10, MCP-1, and IP-10 were higher in patients with high viral loads. The levels of IFN-α, IFN-γ, IL-6, MCP-1, and IP-10 positively correlated with SFTSV viral load.
CONCLUSIONS: SFTSV RNA load was significantly correlated with the concentration of IFN-α, as well as other cytokines (IFN-γ, IL-6, MIP-1α, and IP-10).
METHODS: Viral RNA was isolated from plasma collected in EDTA and quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR). Cytokines in the sera of SFTS patients were measured using cytometric bead array (CBA).
RESULTS: Viral loads ranged from 2.3 × 103 copies/mL to 7.9 × 1011 copies/mL. Of the 46 patients, 10 (21.7%) died during treatment. Viral loads were ≥1 × 108 copies/mL in the patients who died. The concentrations of G-CSF, IFN-α, IFN-γ, IL-1β, IL-6, IL-10, MCP-1, and IP-10 were higher in patients with high viral loads. The levels of IFN-α, IFN-γ, IL-6, MCP-1, and IP-10 positively correlated with SFTSV viral load.
CONCLUSIONS: SFTSV RNA load was significantly correlated with the concentration of IFN-α, as well as other cytokines (IFN-γ, IL-6, MIP-1α, and IP-10).