Evidence of Neospora caninum infection in buffaloes (Bubalus bubalis) from Northwestern Romania.

Neospora caninum is a heteroxenous parasitic Apicomplexan protozoan, able to infect a variety of domestic and wild animals, mainly associated with reproductive disorders and abortions in susceptible species. Considering the importance of Romania in the European buffalo industry and the severe economic losses caused by N. caninum infection in livestock, the aim of the present study was to assess the occurrence of neosporosis in buffaloes raised in household and farming systems in northwestern Romania. Overall, 197 serum samples were tested for the presence of N. caninum-specific antibodies by ELISA. Additionally, from 74 slaughtered buffaloes, diaphragm digest, heart, and lymph node samples were tested for the presence of N. caninum DNA. The overall seroprevalence was 68.5%. The seroprevalence was significantly higher in adult animals compared to calves and young ones and in the household system compared to farm. By nPCR, six samples were found to be positive for N. caninum DNA, of which three were serologically negative. The present study reveals a high prevalence of N. caninum-specific antibodies in buffaloes in northwestern Romania and to our best knowledge, it is the first one performed in Romania.