Is ovarian adrenergic innervation essential to gonadal function in adult rats?

We studied the relative importance of ovarian innervation during different phases of female rat sexual development, 30, 40, 45 and 60-day-old. Chemical sympathectomy was promoted by long term postnatal treatment with guanethidine (GD), an adrenergic neuron blocking agent. The sympathectomized rats exhibited delayed puberty and alterations in estrous cycle. Reduced circulating E2, LH and Prl levels at 30 days of age were observed, besides decreased steroidal secretory responsiveness to gonadotropins in vitro. On the other hand, normal pituitary secretory responsiveness to LHRH in vivo and plasma gonadotropin concentrations were observed in 60-day-old rats. These data reinforce the view that ovarian sympathetic innervation contributes to the regulation of the prepubertal development of the ovarian functions.