Haplotype-resolved genome sequences of a springtail species, Folsomia candida (Collembola: Isotomidae).

Folsomia candida (Collembola: Isotomidae) is one of the most important indicator species for soil animal studies and is widely distributed worldwide. Here, we utilized PacBio HiFi long reads, Oxford Nanopore Technologies (ONT) ultralong reads, Hi-C, Illumina short reads, and ONT long-read transcriptome sequencing to assemble and annotate the genome of Folsomia candida. Two gapless, telomere-to-telomere, haplotype-resolved genomes (HapA and HapB) were successfully assembled, with genome sizes of 228.62 and 228.45 Mb, respectively. The scaffold N50 sizes are 41.45 Mb for both haplotypes. We manually curated and annotated each gene on HapA, providing comprehensive annotation information for this haplotype. Gene annotation revealed a total of 26,329 protein-coding genes in HapA and 26,768 in HapB. These haplotype-resolved Folsomia candida genomes provide a high-quality genomic resource that enables future studies on soil arthropods and springtail genomics.