Antibiotic control of tissue reactions in dogs vaccinated with viable cells of Coccidioides immitis.

Castleberry, Merida W. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), John L. Converse, and Peter J. Soto, Jr. Antibiotic control of tissue reactions in dogs vaccinated with viable cells of Coccidioides immitis. J. Bacteriol. 87:1216-1220. 1964.-A total of 12 dogs (15 to 25 lb each), vaccinated with viable Coccidioides immitis (subcutaneous injection of 260 viable arthrospores in the medial surface of the hind leg), resisted a respiratory challenge (aerosol) with the same organism (13,000 viable arthrospores) administered (aerosol) 2 months after vaccination. Oral amphotericin B therapy (150 mg of Fungizone per day for 21 days) of 6 of the 12 dogs, initiated immediately after vaccination, eliminated the undesirable side reactions of the viable vaccine (ulcerated vaccination site and inguinal lymphadenopathy exhibited by the 6 untreated dogs) without affecting the immunogenicity of the vaccine. Clinical observation (blood-urea nitrogen levels) during and after therapy and histological examination approximately 3 months after respiratory challenge failed to disclose any evidence of nephrotoxicity or renal damage due to the oral antibiotic therapy (total doses of more than 3 g of amphotericin B).