International journal of clinical and experimental medicine · 2015
Preliminary study of the effect of abnormal savda munziq on TGF-β1 and Smad7 expression in hypertrophic scar fibroblasts.
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Wang Hujun, Gao Weicheng, Kong Menglong, Li Nan, Ma Shaolin
Аннотация
BACKGROUND: To study the effect of abnormal savda munziq (ASMq) on TGF-β1 and Smad7 expression in hypertrophic scar fibroblasts (HSFs) and to preliminarily assess the function of abnormal savda munziq in hypertrophic scar formation at the molecular biology level.
METHODS: HSFs were cultured in vitro. RT-PCR and Western-blot were used to investigate the influence of 48-h treatment with ASMq at different concentrations (0 mg/mL, 0.1 mg/mL, 0.4 mg/mL, and 0.7 mg/mL) on TGF-β1 and Smad7 mRNA and protein expression levels.
RESULTS: After 48-h treatment with ASMq, the expression of TGF-β1 mRNA and protein gradually decreased in HSFs as the concentration increased. In contrary, Smad7 mRNA and protein expression were positively correlated with ASMq concentration.
CONCLUSIONS: ASMq reduces TGF-β1, increases Smad7 mRNA and protein expression through regulating TGFβ-1/Smad signaling pathway, inhibiting HSFs proliferation and reducing extracellular collagen deposition.
METHODS: HSFs were cultured in vitro. RT-PCR and Western-blot were used to investigate the influence of 48-h treatment with ASMq at different concentrations (0 mg/mL, 0.1 mg/mL, 0.4 mg/mL, and 0.7 mg/mL) on TGF-β1 and Smad7 mRNA and protein expression levels.
RESULTS: After 48-h treatment with ASMq, the expression of TGF-β1 mRNA and protein gradually decreased in HSFs as the concentration increased. In contrary, Smad7 mRNA and protein expression were positively correlated with ASMq concentration.
CONCLUSIONS: ASMq reduces TGF-β1, increases Smad7 mRNA and protein expression through regulating TGFβ-1/Smad signaling pathway, inhibiting HSFs proliferation and reducing extracellular collagen deposition.