Медицинская библиотека · Cell stem cell · 2022

A reference human induced pluripotent stem cell line for large-scale collaborative studies.

Pantazis Caroline B, Yang Andrian, Lara Erika, McDonough Justin A, Blauwendraat Cornelis, Peng Lirong, Oguro Hideyuki, Kanaujiya Jitendra, Zou Jizhong, Sebesta David, Pratt Gretchen, Cross Erin, Blockwick Jeffrey, Buxton Philip, Kinner-Bibeau Lauren, Medura Constance, Tompkins Christopher, Hughes Stephen, Santiana Marianita, Faghri Faraz, Nalls Mike A, Vitale Daniel, Ballard Shannon, Qi Yue A, Ramos Daniel M, Anderson Kailyn M, Stadler Julia, Narayan Priyanka, Papademetriou Jason, Reilly Luke, Nelson Matthew P, Aggarwal Sanya, Rosen Leah U, Kirwan Peter, Pisupati Venkat, Coon Steven L, Scholz Sonja W, Priebe Theresa, Öttl Miriam, Dong Jian, Meijer Marieke, Janssen Lara J M, Lourenco Vanessa S, van der Kant Rik, Crusius Dennis, Paquet Dominik, Raulin Ana-Caroline, Bu Guojun, Held Aaron, Wainger Brian J, Gabriele Rebecca M C, Casey Jackie M, Wray Selina, Abu-Bonsrah Dad, Parish Clare L, Beccari Melinda S, Cleveland Don W, Li Emmy, Rose Indigo V L, Kampmann Martin, Calatayud Aristoy Carles, Verstreken Patrik, Heinrich Laurin, Chen Max Y, Schüle Birgitt, Dou Dan, Holzbaur Erika L F, Zanellati Maria Clara, Basundra Richa, Deshmukh Mohanish, Cohen Sarah, Khanna Richa, Raman Malavika, Nevin Zachary S, Matia Madeline, Van Lent Jonas, Timmerman Vincent, Conklin Bruce R, Johnson Chase Katherine, Zhang Ke, Funes Salome, Bosco Daryl A, Erlebach Lena, Welzer Marc, Kronenberg-Versteeg Deborah, Lyu Guochang, Arenas Ernest, Coccia Elena, Sarrafha Lily, Ahfeldt Tim, Marioni John C, Skarnes William C, Cookson Mark R, Ward Michael E, Merkle Florian T

СтатьяResearch Support, N.I.H., IntramuralПоддержка исследования: вне правительства СШАПоддержка NIH: внешняя программаПеревод пока не подготовлен

Аннотация

Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field.

Для цитирования: Pantazis Caroline B, Yang Andrian, Lara Erika, McDonough Justin A, Blauwendraat Cornelis, Peng Lirong, Oguro Hideyuki, Kanaujiya Jitendra, Zou Jizhong, Sebesta David, Pratt Gretchen, Cross Erin, Blockwick Jeffrey, Buxton Philip, Kinner-Bibeau Lauren, Medura Constance, Tompkins Christopher, Hughes Stephen, Santiana Marianita, Faghri Faraz, Nalls Mike A, Vitale Daniel, Ballard Shannon, Qi Yue A, Ramos Daniel M, Anderson Kailyn M, Stadler Julia, Narayan Priyanka, Papademetriou Jason, Reilly Luke, Nelson Matthew P, Aggarwal Sanya, Rosen Leah U, Kirwan Peter, Pisupati Venkat, Coon Steven L, Scholz Sonja W, Priebe Theresa, Öttl Miriam, Dong Jian, Meijer Marieke, Janssen Lara J M, Lourenco Vanessa S, van der Kant Rik, Crusius Dennis, Paquet Dominik, Raulin Ana-Caroline, Bu Guojun, Held Aaron, Wainger Brian J, Gabriele Rebecca M C, Casey Jackie M, Wray Selina, Abu-Bonsrah Dad, Parish Clare L, Beccari Melinda S, Cleveland Don W, Li Emmy, Rose Indigo V L, Kampmann Martin, Calatayud Aristoy Carles, Verstreken Patrik, Heinrich Laurin, Chen Max Y, Schüle Birgitt, Dou Dan, Holzbaur Erika L F, Zanellati Maria Clara, Basundra Richa, Deshmukh Mohanish, Cohen Sarah, Khanna Richa, Raman Malavika, Nevin Zachary S, Matia Madeline, Van Lent Jonas, Timmerman Vincent, Conklin Bruce R, Johnson Chase Katherine, Zhang Ke, Funes Salome, Bosco Daryl A, Erlebach Lena, Welzer Marc, Kronenberg-Versteeg Deborah, Lyu Guochang, Arenas Ernest, Coccia Elena, Sarrafha Lily, Ahfeldt Tim, Marioni John C, Skarnes William C, Cookson Mark R, Ward Michael E, Merkle Florian T. A reference human induced pluripotent stem cell line for large-scale collaborative studies.. Cell stem cell. 2022. DOI: 10.1016/j.stem.2022.11.004.

Предметные рубрики MeSH

Humans Induced Pluripotent Stem Cells Cell Differentiation Gene Editing Biological Assay

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