Effect of local injection of injectable platelet-rich fibrin on eruption rate of teeth (Rabbit animal model).

OBJECTIVE

This study aimed to evaluate the effect of the local injection of injectable platelet-rich fibrin (i-PRF) on the eruption rate of the teeth and evaluation of the effect of i-PRF on the number of odontoblast, cementoblast, osteoblast, osteocyte, and osteoclast cells.

MATERIALS AND

METHODS

The samples consisted of 20 male albino rabbits and were divided randomly into the control group (5 rabbits) and i-PRF groups (15 rabbits) sub-divided into week 1, week 2, and week 3 sub-groups. The labial surface of lower right incisors was marked by drilling a hole at the level of the free gingival margin in the mid-line of each tooth with a small round bur, and the eruption rates were measured by measuring the distance from the most apical point of the free gingival margin and the center of the hole with a digital caliper. i-PRF was prepared by centrifuging autologous blood of each rabbit at 1000 rpm for 5 minutes; then the i-PRF layer was collected. The control group received no treatment, whereas i-PRF groups received i-PRF in the following manner: For week 1 groups, a single dose was given at 0 day; for week 2 groups, double doses were given at 0 and 7 days; and for week 3 groups, three doses were given at 0, 7, and 14 days. At the end of experiments for each group, animal scarification was performed, and histological steps were performed.

RESULTS

There was a significant increase in the rate of tooth eruption in i-PRF groups when compared to the control group, and the eruption rate was increased in 107.3%, 144.7%, and 167.5% for week 1, week 2, and week 3, respectively. Concerning the histological finding, the i-PRF groups gave rise to a higher number of odontoblast, cementoblast, osteoblast, osteocyte, and osteoclast cells with a significant difference when compared to the control groups (P ≤ 0.05).

CONCLUSIONS

Applying i-PRF significantly increased the rate of teeth eruption at all-time intervals. Platelet-rich fibrin injection can be an effective method for acceleration of teeth eruption.