Method for needle-free release of pathogens from host cells using reusable chromatography components.

Release of intracellular pathogens from host cells is frequently performed by forcing the cells through hypodermic needles attached to a syringe ("syringing"). This can result in needle sticks and infectious aerosols. We describe a reusable, economical host cell lysis device assembled from chromatography components for needle-free and aerosol-minimized manual cell disruption.