A novel bioactive and functional exopolysaccharide from the cyanobacterial strain Arthrospira maxima cultivated under salinity stress.

Cyanobacterial exopolysaccharides (EPS) remain released by cyanobacteria in the surrounding environment with the main purpose of protection against harmful environmental conditions. Recently, they have received significant attention due to their unique structural characteristics, functional properties, and potential applications across various fields. The current study describes the evaluation of EPS production under salinity stress from Arthrospira maxima. The application of high salinity up to 40 g/L enhanced EPS production, which was collected and purified by alcohol precipitation followed by membrane dialysis and lyophilization. A yield of 60 mg/L was obtained. The Size exclusion chromatography gave for the purified EPS an apparent molecular weight of 2.1 × 105 Da. Monosaccharide composition showed that EPS is a heteropolymer, with mannose, xylose, and glucuronic acid identified as the predominant monosaccharides and derivatives. Nuclear magnetic resonance spectroscopy (13C and 1H) confirmed that EPS is a heteropolysaccharide, entirely in α- anomeric configuration, with glucuronic acid as a main monomer that is probably linked to mannose and xylose via α-glycosidic linkages. Bioactivity assessment of EPS revealed that it exhibits antibacterial activity against several strains, notably, Bacillus subtilis (MIC: 0.6 ± 0.05 mg/mL), Bacillus cereus (MIC: 1 ± 0.01 mg/mL), Escherichia coli (MIC: 0.8 ± 0.01 mg/mL) and Klebsiella pneumonia (MIC: 0.8 ± 0.01 mg/mL). Antioxidant activity was measured using the DPPH radical scavenging assay, yielding an IC₅₀ of 6.83 mg/mL. Besides, EPS was also found to exhibit an interesting emulsifying property with several oil types, indicating its potential as a versatile biopolymer for applications in various industrial sectors.