Contact dermatitis · 2012
Stability of selected volatile contact allergens in different patch test chambers under different storage conditions.
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Mose Kristian F, Andersen Klaus E, Christensen Lars Porskjaer
Аннотация
BACKGROUND: Patch test preparations of volatile substances may evaporate during storage, thereby giving rise to reduced patch test concentrations.
OBJECTIVES: To investigate the stability of selected acrylates/methacrylates and fragrance allergens in three different test chambers under different storage conditions.
METHODS: Petrolatum samples of methyl methacrylate (MMA), 2-hydroxyethyl methacrylate (2-HEMA), 2-hydroxypropyl acrylate (2-HPA), cinnamal and eugenol in patch test concentrations were stored in three different test chambers (IQ chamber™, IQ Ultimate™, and Van der Bend® transport container) at room temperature and in a refrigerator. The samples were analysed in triplicate with high-performance liquid chromatography.
RESULTS: The decrease in concentration was substantial for all five allergens under both storage conditions in IQ chamber™ and IQ Ultimate™, with the exception of 2-HEMA during storage in the refrigerator. For these two chamber systems, the contact allergen concentration dropped below the stability limit in the following order: MMA, cinnamal, 2-HPA, eugenol, and 2-HEMA. In the Van der Bend® transport container, the contact allergens exhibited acceptable stability under both storage conditions, whereas MMA and 2-HPA required cool storage for maintenance of the limit.
CONCLUSION: The Van der Bend® transport container was the best device for storage of samples of volatile contact allergens.
OBJECTIVES: To investigate the stability of selected acrylates/methacrylates and fragrance allergens in three different test chambers under different storage conditions.
METHODS: Petrolatum samples of methyl methacrylate (MMA), 2-hydroxyethyl methacrylate (2-HEMA), 2-hydroxypropyl acrylate (2-HPA), cinnamal and eugenol in patch test concentrations were stored in three different test chambers (IQ chamber™, IQ Ultimate™, and Van der Bend® transport container) at room temperature and in a refrigerator. The samples were analysed in triplicate with high-performance liquid chromatography.
RESULTS: The decrease in concentration was substantial for all five allergens under both storage conditions in IQ chamber™ and IQ Ultimate™, with the exception of 2-HEMA during storage in the refrigerator. For these two chamber systems, the contact allergen concentration dropped below the stability limit in the following order: MMA, cinnamal, 2-HPA, eugenol, and 2-HEMA. In the Van der Bend® transport container, the contact allergens exhibited acceptable stability under both storage conditions, whereas MMA and 2-HPA required cool storage for maintenance of the limit.
CONCLUSION: The Van der Bend® transport container was the best device for storage of samples of volatile contact allergens.