Acta tropica · 2026
Oncostatin level and Tumour necrosis factor-alpha gene polymorphism in predicting Blastocystis infection in inflammatory bowel disease.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ibrahim Asmaa, Rageh Fatma, Abdelhady Shaymaa Abdelraheem, Elgamal Rasha, Sakr Mohamed A, Medhat Mohamed, Ahmed Samar S, Yassin Hend A, Kamel Yasmine N, Rashad Mai Hamdy, Salim Almaza Ali, Elhoseeny Mohamed Mahmoud, Othman Amira A A, Mazen Moamen A I, Ramadan Manar Ezzelarab
Аннотация
AIM: This study investigated the prevalence of Blastocystis spp. infection and serum oncostatin M (OSM) levels in patients with inflammatory bowel disease (IBD) and examined their association with Blastocystis subtypes and tumor necrosis factor-alpha (TNF-α) gene polymorphisms.
METHODOLOGY: This case-control study included 300 participants. Stool samples were examined microscopically and molecularly to detect Blastocystis and identify its subtypes. Serum OSM levels were measured using enzyme-linked immunosorbent assay. TNF-α polymorphisms at positions -1031T/C and -308G/A were analyzed using polymerase chain reaction-restriction fragment length polymorphism.
RESULTS: Blastocystis infection was detected in 59.5% of patients with IBD compared with 19.0% of healthy controls (P < 0.0001). Two subtypes, ST1 and ST3, were identified, with ST1 being significantly more common in patients with IBD than in controls (P = 0.009). Serum OSM levels were significantly higher in patients with IBD than in controls and were further elevated in Blastocystis-infected patients with IBD compared with non-infected patients (P < 0.0001). OSM levels were also significantly higher in patients infected with ST1 than in those infected with ST3 (P = 0.0004). Significant associations were observed between the TNF-α -308G/A polymorphism and IBD susceptibility, while a trend toward an association was noted for the -1031T/C polymorphism. Genotype and allele frequencies differed significantly between patients infected with Blastocystis ST1 and those infected with ST3 (P < 0.05).
CONCLUSION: Elevated OSM levels and TNF-α polymorphisms are associated with IBD and Blastocystis ST1 infection and may serve as potential biomarkers for early diagnosis and personalized disease management.
METHODOLOGY: This case-control study included 300 participants. Stool samples were examined microscopically and molecularly to detect Blastocystis and identify its subtypes. Serum OSM levels were measured using enzyme-linked immunosorbent assay. TNF-α polymorphisms at positions -1031T/C and -308G/A were analyzed using polymerase chain reaction-restriction fragment length polymorphism.
RESULTS: Blastocystis infection was detected in 59.5% of patients with IBD compared with 19.0% of healthy controls (P < 0.0001). Two subtypes, ST1 and ST3, were identified, with ST1 being significantly more common in patients with IBD than in controls (P = 0.009). Serum OSM levels were significantly higher in patients with IBD than in controls and were further elevated in Blastocystis-infected patients with IBD compared with non-infected patients (P < 0.0001). OSM levels were also significantly higher in patients infected with ST1 than in those infected with ST3 (P = 0.0004). Significant associations were observed between the TNF-α -308G/A polymorphism and IBD susceptibility, while a trend toward an association was noted for the -1031T/C polymorphism. Genotype and allele frequencies differed significantly between patients infected with Blastocystis ST1 and those infected with ST3 (P < 0.05).
CONCLUSION: Elevated OSM levels and TNF-α polymorphisms are associated with IBD and Blastocystis ST1 infection and may serve as potential biomarkers for early diagnosis and personalized disease management.