Filter paper performance in PCR for cutaneous leishmaniasis diagnosis.

INTRODUCTION

The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis.

METHODS

Lesion scrapings from 48 patients were collected and analyzed for PCR.

RESULTS

PCR with FP detected up to three Leishmania braziliensis promastigotes. Considering the direct search by microscopy or PCR of samples collected in STE buffer as standards, the sensitivity of PCR with FP was 100%.

CONCLUSIONS

FP can be useful for CL diagnosis in remote regions, allowing high sensitivity in the detection of the parasite by PCR.