Future microbiology · 2024
Repurposing approved drugs as potential efflux pump inhibitors in multidrug-resistant Pseudomonas aeruginosa.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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de Melo Guedes Gláucia Morgana, Pereira Vinicius Carvalho, Freitas Alyne Soares, Honório de Souza Paulo Roberto, Chacon Parra Aura Lucia, Brasil Jaiane Alves, de Medeiros Guedes Rodrigo Fonseca, Pereira de Sousa Paulo César, Aguiar Cordeiro Rossana de, Gadelha Rocha Marcos Fábio, Costa Sidrim José Júlio, Souza Collares Maia Castelo Branco Débora de
Аннотация
Aim: To evaluate the action of promethazine, fluoxetine and carbonyl cyanide 3-chlorophenylhydrazone as efflux pump inhibitors (EPIs) against multidrug-resistant Pseudomonas aeruginosa. Methods: The effect of the compounds was evaluated in planktonic cells and bacterial biofilms. Accumulation tests were performed with ethidium bromide to prove their action as EPIs. Then, they were associated with antimicrobials. Results: Effect on planktonic cells and biofilms was found. Assays with ethidium bromide indicate their action as EPIs. Significant reductions in the metabolic activity of biofilms were observed after the association with the antimicrobials, especially for meropenem. Conclusion: It is possible to prove the action of these compounds as EPIs for P. aeruginosa and demonstrate the relevance of efflux pumps in antimicrobial resistance.