Медицинская библиотека · American journal of ophthalmology · 2005

The genotype of Toxoplasma gondii strains causing ocular toxoplasmosis in humans in Brazil.

Vallochi Adriana Lima, Muccioli Cristina, Martins Maria Cristina, Silveira Cláudio, Belfort Rubens, Rizzo Luiz Vicente

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Аннотация

PURPOSE: To determine Toxoplasma gondii genotype in scars of retinochoroiditis in humans.

DESIGN

Immunopathology analysis.

METHODS

DNA was isolated from retinochoroidal specimens taken from the borders of the scars from eye bank eyes as well as enucleated eyes from uveitis patients with retinochoroidal toxoplasmic scars. The SAG2 region was amplified by polymerase chain reaction and analyzed by restriction fragment length polymorphism.

RESULTS

Of the 92 eyes studied, 9 had toxoplasmic scars. All of them, as well as the 2 enucleated eyes, disclosed the presence of type I parasites by the gel electrophoresis analysis of the restriction fragment length polymorphism from SAG2.

CONCLUSION

These results suggest that type I strain seems to be responsible for the ocular infections in the population studied.

Для цитирования: Vallochi Adriana Lima, Muccioli Cristina, Martins Maria Cristina, Silveira Cláudio, Belfort Rubens, Rizzo Luiz Vicente. The genotype of Toxoplasma gondii strains causing ocular toxoplasmosis in humans in Brazil.. American journal of ophthalmology. 2005. DOI: 10.1016/j.ajo.2004.07.040.

Предметные рубрики MeSH

Animals Antigens, Protozoan Brazil Chorioretinitis DNA, Protozoan Eye Banks Eye Enucleation Genotype Humans Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Protozoan Proteins Toxoplasma Toxoplasmosis, Ocular

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