[Zhonghua yan ke za zhi] Chinese journal of ophthalmology · 2007
[Expression of cyclooxygenase-2 in pterygium and effects of selective cyclooxygenase-2 inhibitor on human pterygium fibroblasts].
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Gao Yuan, Zhang Ming-Chang, Peng Yun
Аннотация
OBJECTIVE: To investigate the expression of cyclooxygenase-2 (COX-2) in pterygium and the effect of selective COX-2 inhibitor (Nimesulide) on proliferation of human pterygium fibroblasts (HPF) in culture.
METHODS: Samples of pterygium and conjunctiva were obtained from patients in the Department of Ophthalmology in Union Hospital of Wuhan. The expression of COX-2 in pterygium was determined by immunohistochemistry. The HPF cells were isolated, cultured and identified. Expression of cyclooxygenase-2 was detected by Western blot in HPF following basic fibroblast growth factor (bFGF) treatment at different concentrations (1, 10, 100 ng/ml). The MTT method was used to assay the biologic activities of Nimesulide in different doses (0, 25, 50, 100, 200 micromol/L) and different time (24, 48, 72, 96 h).
RESULTS: The positive rate of expression of COX-2 was 70%. The expression of COX-2 became more significantly as dose increased with the stimulation of bFGF. Administration of 50 micromol/L Nimesulide for 24 h could significantly inhibit HPF proliferation in a dose- and time-dependent manner (P < 0.05).
CONCLUSIONS: bFGF can induce the expression of COX-2 in HPF, COX-2 may be one of factors involved in the formation of pterygium. Nimesulide can significantly inhibit HPF proliferation.
METHODS: Samples of pterygium and conjunctiva were obtained from patients in the Department of Ophthalmology in Union Hospital of Wuhan. The expression of COX-2 in pterygium was determined by immunohistochemistry. The HPF cells were isolated, cultured and identified. Expression of cyclooxygenase-2 was detected by Western blot in HPF following basic fibroblast growth factor (bFGF) treatment at different concentrations (1, 10, 100 ng/ml). The MTT method was used to assay the biologic activities of Nimesulide in different doses (0, 25, 50, 100, 200 micromol/L) and different time (24, 48, 72, 96 h).
RESULTS: The positive rate of expression of COX-2 was 70%. The expression of COX-2 became more significantly as dose increased with the stimulation of bFGF. Administration of 50 micromol/L Nimesulide for 24 h could significantly inhibit HPF proliferation in a dose- and time-dependent manner (P < 0.05).
CONCLUSIONS: bFGF can induce the expression of COX-2 in HPF, COX-2 may be one of factors involved in the formation of pterygium. Nimesulide can significantly inhibit HPF proliferation.