Clinical Utility and Cost-Effectiveness of Pretreatment NUDT15 Pharmacogenetic Testing to Prevent Thiopurine-Induced Myelosuppression: A Genotype-First Reverse Phenotyping Cohort Study Within the UK NIHR Inflammatory Bowel Disease Bioresource.
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Roberts Christopher, Peters Jaime, Sazonvos Aleksejs, Goodman Neil, Sharip Mohmmed, Smith Rebecca, Bishara Maria, Bewshea Claire, Lin Simeng, Chanchlani Neil, Hodges Phoebe, Badrulhisham Fakhirah, Saifuddin Aamir, Carlson Sean, Centritto Andrea, Marley Alexandra, Saad Muhammad, Sethi-Aora Karishma, White Laura, Abdelmeguid Alaa, Pele Laetitia, Sebastian Shaji, Selinger Christian, Irving Peter, Fachal Laura, Walker Gareth, Palmer Rachel, Kennedy Nick, Houghton Jayne, Doona Mary, Lamb Christopher, Hyde Chris, Parkes Miles, Goodhand James, Ahmad Tariq
Аннотация
AIMS: To report the prevalence, penetrance, expressivity, and pathogenicity of NUDT15 variant allele carriage and the diagnostic accuracy and cost-effectiveness of an inexpensive loop-mediated isothermal amplification (LAMP) test.
METHODS: Retrospective case-matched cohort study of rates of severe myelosuppression in patients with and without loss-of-function NUDT15 variant allele(s) treated with a thiopurine.
RESULTS: Overall, 1.3% of Europeans and 11.7% of South Asians carried a variant allele. Severe myelosuppression was associated with NUDT15 variant allele carriage (11.3% vs 0.95%; p < 0.001). Carriage of a single *3, *6 or *9 variant allele was associated with a shorter time to severe myelosuppression. Numbers needed to genotype to prevent myelosuppression in Europeans and South Asians were 786 and 23. Variant calling using the LAMP assay was fully concordant with Sanger sequencing (n = 154). It improved the safety of thiopurine dosing and was cost-effective when used to reduce the frequency and cost of thiopurine blood monitoring for patients without risk variants.
CONCLUSION: We recommend TPMT and NUDT15 genetic testing in patients of Asian and admixed ancestry. In Europeans, reflex NUDT15 testing should be considered in patients with reduced TPMT activity or loss-of-function genotype. Thiopurines should be avoided in patients with > 1 NUDT15 variant allele and in patients with both NUDT15 and TPMT variant alleles. In patients with a single NUDT15 variant allele, we recommend thiopurine dose reduction (< 1 mg/kg/day) and intensified blood test monitoring.