Clinica chimica acta; international journal of clinical chemistry · 2026
Dot-ELISA as a diagnostic tool: Detection of anti-Strongyloides antibodies in serum and saliva from donors at a regional blood Center in Northern Paraná.
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Dos Santos Destro Yasmin Munhoz, Suzuki Sara Mayumi, Corral Marcelo Andreetta, Nunes Angélica Paulina, Rossi Izadora Volpato, Lima Dirce Mary Correia, Vasconcelos Allana Martins, Nunes Cicero Monael Dias Pessoa, Roldán William Henry, Trigo Fausto Celso, Gryschek Ronaldo Cesar Borges, de Paula Fabiana Martins, Costa Idessania Nazareth
Аннотация
BACKGROUND: Strongyloidiasis, caused by Strongyloides stercoralis, remains underdiagnosed due to limitations of conventional parasitological methods, including intermittent larval shedding and low sensitivity in chronic and asymptomatic infections. This study aimed to evaluate the diagnostic performance of a Dot-ELISA assay for the detection of anti-S. stercoralis antibodies in serum and saliva samples from blood donors.
METHODS: A total of 58 serum samples and 75 saliva samples from individuals previously classified by IgG ELISA and IgG Western blotting using heterologous Strongyloides venezuelensis antigen were analyzed. The diagnostic performance of Dot-ELISA was assessed using receiver operating characteristic (ROC) curves, sensitivity, and specificity, and results were compared with established serological methods.
RESULTS: Dot-ELISA demonstrated high discriminatory ability between positive and negative serum samples and good performance in saliva samples, with results in agreement with ELISA and Western blotting. The assay successfully detected anti-S. stercoralis antibodies in both biological matrices, indicating reliable agreement with reference methods. The use of saliva as a biological sample represents a less invasive and more practical alternative for antibody detection.
CONCLUSION: Dot-ELISA is a simple, low-cost method with reliable diagnostic performance for detecting S. stercoralis infection in both serum and saliva samples. Its application may improve access to diagnosis and support screening and surveillance strategies, particularly in endemic regions and settings with limited laboratory infrastructure.
METHODS: A total of 58 serum samples and 75 saliva samples from individuals previously classified by IgG ELISA and IgG Western blotting using heterologous Strongyloides venezuelensis antigen were analyzed. The diagnostic performance of Dot-ELISA was assessed using receiver operating characteristic (ROC) curves, sensitivity, and specificity, and results were compared with established serological methods.
RESULTS: Dot-ELISA demonstrated high discriminatory ability between positive and negative serum samples and good performance in saliva samples, with results in agreement with ELISA and Western blotting. The assay successfully detected anti-S. stercoralis antibodies in both biological matrices, indicating reliable agreement with reference methods. The use of saliva as a biological sample represents a less invasive and more practical alternative for antibody detection.
CONCLUSION: Dot-ELISA is a simple, low-cost method with reliable diagnostic performance for detecting S. stercoralis infection in both serum and saliva samples. Its application may improve access to diagnosis and support screening and surveillance strategies, particularly in endemic regions and settings with limited laboratory infrastructure.