Revista da Sociedade Brasileira de Medicina Tropical · 2019
IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Gusmão Michélia Antônia do Nascimento, Emídio Nayara Braga, Marconato Danielle Gomes, Farani Priscila Silva Grijó, Maniezzi Luiz Felipe, Coelho Paulo Marcos Zech, Oliveira Rodrigo Correa, Vasconcelos Eveline Gomes, Faria-Pinto Priscila de
Аннотация
INTRODUCTION: High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins.
METHODS: Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity.
RESULTS: Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production.
CONCLUSIONS: Potato apyrase and SmATPDases have IgE-binding epitopes.
METHODS: Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity.
RESULTS: Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production.
CONCLUSIONS: Potato apyrase and SmATPDases have IgE-binding epitopes.