Revista chilena de infectologia : organo oficial de la Sociedad Chilena de Infectologia · 2012
[Morphological and molecular identification of canine filariae in a semi-rural district of the Metropolitan Region in Chile].
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López Javier, Valiente-Echeverría Fernando, Carrasco Marcela, Mercado Rubén, Abarca Katia
Аннотация
INTRODUCTION: To date, there has been no definitive confirmation of the presence of zoonotic dirofilariasis in dogs in Chile.
OBJECTIVES: To study the presence of dirofilarias in blood samples from dogs collected in a semi-rural district near Santiago and to compare their frequency in dogs with and without dermatological manifestations.
METHODS: We examined 100 blood samples for dog filariae infections using microscopic methods (modified Knott technique). 50 dogs presented dermatological symptoms or signs compatible with filarial infections and 50 were asymptomatic. ITS-2 and 12s rDNA gene amplification by PCR and sequencing were performed in samples microscopically positive for microfilariae. Results. We observed microfilariae in 22 dogs (22%). Of these, 16/50 (32%) were symptomatic and 6/50 (12%) were asymptomatic (p = 0.02). Morphologically, the majority of micro-filariae were similar to Dirofilaria repens, although many had a bigger size than previously described. Nucleotide sequencing of the amplified genes showed no more than 95% homology with the D. repens sequences available for comparison. D. reconditum and D. dracunculoides infections were also identified.
CONCLUSIONS: These features might indicate the presence of new species of Dirofilaria or a D. repens close related variant in Chile.
OBJECTIVES: To study the presence of dirofilarias in blood samples from dogs collected in a semi-rural district near Santiago and to compare their frequency in dogs with and without dermatological manifestations.
METHODS: We examined 100 blood samples for dog filariae infections using microscopic methods (modified Knott technique). 50 dogs presented dermatological symptoms or signs compatible with filarial infections and 50 were asymptomatic. ITS-2 and 12s rDNA gene amplification by PCR and sequencing were performed in samples microscopically positive for microfilariae. Results. We observed microfilariae in 22 dogs (22%). Of these, 16/50 (32%) were symptomatic and 6/50 (12%) were asymptomatic (p = 0.02). Morphologically, the majority of micro-filariae were similar to Dirofilaria repens, although many had a bigger size than previously described. Nucleotide sequencing of the amplified genes showed no more than 95% homology with the D. repens sequences available for comparison. D. reconditum and D. dracunculoides infections were also identified.
CONCLUSIONS: These features might indicate the presence of new species of Dirofilaria or a D. repens close related variant in Chile.