PloS one · 2012
Novel insights into the transcriptome of Dirofilaria immitis.
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Fu Yan, Lan Jingchao, Zhang Zhihe, Hou Rong, Wu Xuhang, Yang Deying, Zhang Runhui, Zheng Wanpeng, Nie Huaming, Xie Yue, Yan Ning, Yang Zhi, Wang Chengdong, Luo Li, Liu Li, Gu Xiaobin, Wang Shuxian, Peng Xuerong, Yang Guangyou
Аннотация
BACKGROUND: The heartworm Dirofilaria immitis is the causal agent of cardiopulmonary dirofilariosis in dogs and cats, and also infects a wide range of wild mammals as well as humans. One bottleneck for the design of fundamentally new intervention and management strategies against D. immitis may be the currently limited knowledge of fundamental molecular aspects of D. immitis.
METHODOLOGY/PRINCIPAL FINDINGS: A next-generation sequencing platform combining computational approaches was employed to assess a global view of the heartworm transcriptome. A total of 20,810 unigenes (mean length = 1,270 bp) were assembled from 22.3 million clean reads. From these, 15,698 coding sequences (CDS) were inferred, and about 85% of the unigenes had orthologs/homologs in public databases. Comparative transcriptomic study uncovered 4,157 filarial-specific genes as well as 3,795 genes potentially involved in filarial-Wolbachia symbiosis. In addition, the potential intestine transcriptome of D. immitis (1,101 genes) was mined for the first time, which might help to discover 'hidden antigens'.
CONCLUSIONS/SIGNIFICANCE: This study provides novel insights into the transcriptome of D. immitis and sheds light on its molecular processes and survival mechanisms. Furthermore, it provides a platform to discover new vaccine candidates and potential targets for new drugs against dirofilariosis.
METHODOLOGY/PRINCIPAL FINDINGS: A next-generation sequencing platform combining computational approaches was employed to assess a global view of the heartworm transcriptome. A total of 20,810 unigenes (mean length = 1,270 bp) were assembled from 22.3 million clean reads. From these, 15,698 coding sequences (CDS) were inferred, and about 85% of the unigenes had orthologs/homologs in public databases. Comparative transcriptomic study uncovered 4,157 filarial-specific genes as well as 3,795 genes potentially involved in filarial-Wolbachia symbiosis. In addition, the potential intestine transcriptome of D. immitis (1,101 genes) was mined for the first time, which might help to discover 'hidden antigens'.
CONCLUSIONS/SIGNIFICANCE: This study provides novel insights into the transcriptome of D. immitis and sheds light on its molecular processes and survival mechanisms. Furthermore, it provides a platform to discover new vaccine candidates and potential targets for new drugs against dirofilariosis.
Предметные рубрики MeSH
AnimalsCluster AnalysisComputational BiologyDatabases, GeneticDirofilaria immitisDogsExpressed Sequence TagsFemaleGene Expression RegulationGenes, HelminthHigh-Throughput Nucleotide SequencingHost-Parasite InteractionsIntestinesMaleMolecular Sequence AnnotationMolecular Sequence DataRNA, MessengerSequence Homology, Nucleic AcidTranscriptome