The Journal of infectious diseases · 2026
Genetic Evidence of Cross-Border Plasmodium vivax Spread in a Malaria Pre-elimination Region of South Asia.
СтатьяНазвание и аннотация: Перевод в очереди
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Rai Anjana, Sutanto Edwin, Ghimire Prakash, Wangchuk Sonam, Drukpa Tobgyel, Alam Mohammad Shafiul, Ghanchi Najia, Rahim Awab Gulam, Rumaseb Angela, Trimarsanto Hidayat, Hoon Kian Soon, Adhikari Nabaraj, Adhikari Sanjib, Banjara Megha Raj, Rijal Komal Raj, Nepal Roshan, Regmi Ramesh Sharma, Qurashi Bushra, Zaidi Shan-E-Zehra, Beg Mohammad Asim, Ley Benedikt, Price Ric N, Thriemer Kamala, Auburn Sarah
Аннотация
BACKGROUND: Plasmodium vivax is the predominant cause of malaria in South Asia. P. vivax cases have fallen over the past decade, but cross-border transmission remains a major challenge to elimination. Genetic data can generate valuable insights into transmission; however, until now, only low-resolution data have been available from Nepal, Bhutan and Bangladesh. We piloted high-resolution genotyping using a new microhaplotype (multiallelic) assay to monitor P. vivax transmission across borders.
METHODS: Genotyping was conducted using the 93-microhaplotype vivaxGEN panel on P. vivax parasites collected from patients enrolled in clinical trials in Bangladesh, Bhutan, and Nepal between 2013 and 2023. These data were compared with open-access microhaplotype and genomic data derived from Afghanistan, India, and Pakistan between 2014 and 2024. Complexity and relatedness (identity by descent [IBD]) were determined within and between countries.
RESULTS: High-quality genotyping data were generated for Nepal (n = 19), Bhutan (n = 27), and Bangladesh (n = 35); comparative data were sourced from Afghanistan (n = 159), India (n = 24), and Pakistan (n = 213). High-complexity infections were observed in 27.04% (43/159) of isolates from Afghanistan, 16.43% (35/213) from Pakistan, and 28.57% (10/35) from Bangladesh. Low prevalence of high-complexity infections was observed in Bhutan (3.70%; 1/27) and Nepal (5.26%; 1/19), suggesting lower superinfection or co-infection. Country-wide IBD analyses revealed 3 genetic clusters partitioning Bangladesh and Bhutan (partial) from the remaining countries. There were 2 subpopulations in Bhutan, which separated local and cross-border imported cases.
CONCLUSIONS: Our results highlight the use of regional high-resolution genetic data to enhance monitoring of transmission intensity and cross-border importations.
METHODS: Genotyping was conducted using the 93-microhaplotype vivaxGEN panel on P. vivax parasites collected from patients enrolled in clinical trials in Bangladesh, Bhutan, and Nepal between 2013 and 2023. These data were compared with open-access microhaplotype and genomic data derived from Afghanistan, India, and Pakistan between 2014 and 2024. Complexity and relatedness (identity by descent [IBD]) were determined within and between countries.
RESULTS: High-quality genotyping data were generated for Nepal (n = 19), Bhutan (n = 27), and Bangladesh (n = 35); comparative data were sourced from Afghanistan (n = 159), India (n = 24), and Pakistan (n = 213). High-complexity infections were observed in 27.04% (43/159) of isolates from Afghanistan, 16.43% (35/213) from Pakistan, and 28.57% (10/35) from Bangladesh. Low prevalence of high-complexity infections was observed in Bhutan (3.70%; 1/27) and Nepal (5.26%; 1/19), suggesting lower superinfection or co-infection. Country-wide IBD analyses revealed 3 genetic clusters partitioning Bangladesh and Bhutan (partial) from the remaining countries. There were 2 subpopulations in Bhutan, which separated local and cross-border imported cases.
CONCLUSIONS: Our results highlight the use of regional high-resolution genetic data to enhance monitoring of transmission intensity and cross-border importations.