Qingshu Yiqi tea prescription attenuates heat-stressed injury: network pharmacology and experimental validation.

Heat stress (HS) poses a significant threat to health, necessitating effective interventions. This study investigated the therapeutic mechanisms of Qingshu Yiqi Tea Prescription (QSYQTP) against HS-induced organ damage in mice. Utilizing an integrated pharmacology approach combining histopathology, network pharmacology, biochemical assays, and qRT-PCR, we assessed QSYQTP's effects in a heat-stressed murine model. QSYQTP supplementation suppressed serum aminotransferase activities (ALT, AST), reduced MDA concentrations, and enhanced antioxidant enzymes (T-SOD, GSH). It also downregulated heat shock genes (HSP27/60/70/90) by inhibiting HSF1 expression, suggesting MAPK involvement, MAPK signaling pathway inhibition (P-JNK, P-P38). These results suggest that QSYQTP may increase the antioxidant capacity in vivo, reduce oxidative stress, and inhibit the heat shock response, all of which can help ameliorate HS-induced liver and small intestine damage.