ACS measurement science au · 2022
Electrochemical Capillary Driven Immunoassay for Detection of SARS-CoV-2.
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Clark Kaylee M, Schenkel Melissa S, Pittman Trey W, Samper Isabelle C, Anderson Loran B R, Khamcharoen Wisarut, Elmegerhi Suad, Perera Rushika, Siangproh Weena, Kennan Alan J, Geiss Brian J, Dandy David S, Henry Charles S
Аннотация
The COVID-19 pandemic focused attention on a pressing need for fast, accurate, and low-cost diagnostic tests. This work presents an electrochemical capillary driven immunoassay (eCaDI) developed to detect SARS-CoV-2 nucleocapsid (N) protein. The low-cost flow device is made of polyethylene terephthalate (PET) and adhesive films. Upon addition of a sample, reagents and washes are sequentially delivered to an integrated screen-printed carbon electrode for detection, thus automating a full sandwich immunoassay with a single end-user step. The modified electrodes are sensitive and selective for SARS-CoV-2 N protein and stable for over 7 weeks. The eCaDI was tested with influenza A and Sindbis virus and proved to be selective. The eCaDI was also successfully applied to detect nine different SARS-CoV-2 variants, including Omicron.