Fertility and sterility · 2001
Histocompatibility leukocyte antigen-G is not expressed by endometriosis or endometrial tissue.
СтатьяПоддержка исследования: вне правительства СШАПоддержка службы здравоохранения СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Hornung D, Fujii E, Lim K H, Vigne J L, McMaster M T, Taylor R N
Аннотация
OBJECTIVE: The immunological mechanisms that support persistence and proliferation of ectopic endometrial implants within the peritoneal cavity of women with endometriosis are unknown. Inhibition of natural killer (NK) and cytotoxic T-cell function has been proposed as a mechanism. We tested the hypothesis that expression of a nonclassical major histocompatibility antigen, HLA-G, might explain the local immunosuppression associated with ectopic endometrium.
DESIGN: Nested case-control study of women with and without laparoscopic evidence of endometriosis.
SETTING: Reproductive endocrinology clinic at a university hospital.
PATIENT(S): Peritoneal fluid specimens from 10 women with revised AFS stage I-IV endometriosis and from 10 age-matched normal controls without laparoscopic evidence of endometriosis were tested for the presence of HLA-G protein. Endometriosis and normal endometrial biopsies from four patients were used to prepare stromal cell cultures directly evaluated for HLA-G protein.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): The expression of HLA-G in peritoneal fluid, tissue, and cell cultures was determined by immunoblotting with a specific monoclonal antibody.
RESULT(S): HLA-G protein was not detectable in peritoneal fluid specimens of endometriosis patients or controls. Moreover, ectopic and normal endometrial tissues and stromal cells did not express HLA-G.
CONCLUSION(S): Immune cell inhibition in endometriosis must be mediated by factors other than HLA-G.
DESIGN: Nested case-control study of women with and without laparoscopic evidence of endometriosis.
SETTING: Reproductive endocrinology clinic at a university hospital.
PATIENT(S): Peritoneal fluid specimens from 10 women with revised AFS stage I-IV endometriosis and from 10 age-matched normal controls without laparoscopic evidence of endometriosis were tested for the presence of HLA-G protein. Endometriosis and normal endometrial biopsies from four patients were used to prepare stromal cell cultures directly evaluated for HLA-G protein.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): The expression of HLA-G in peritoneal fluid, tissue, and cell cultures was determined by immunoblotting with a specific monoclonal antibody.
RESULT(S): HLA-G protein was not detectable in peritoneal fluid specimens of endometriosis patients or controls. Moreover, ectopic and normal endometrial tissues and stromal cells did not express HLA-G.
CONCLUSION(S): Immune cell inhibition in endometriosis must be mediated by factors other than HLA-G.
Предметные рубрики MeSH
Amino Acid SequenceAntibodies, MonoclonalAscitic FluidBlotting, WesternCase-Control StudiesCells, CulturedChoriocarcinomaEndometriosisEndometriumEpitopesFemaleHLA AntigensHLA-G AntigensHistocompatibility Antigens Class IHumansLaparoscopyMolecular Sequence DataReference ValuesStromal CellsTumor Cells, Cultured