Annali di igiene : medicina preventiva e di comunita · 2019
Molecular detection of carbapenem-resistant genes in clinical isolates of Klebsiella pneumoniae.
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Delarampour A, Ghalehnoo Z R, Khademi F, Delarampour M, Vaez H
Аннотация
INTRODUCTION: The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a considerable threat to public health worldwide. The aim of this study was to determine the prevalence of CRKP isolated from clinical specimens by phenotypic and genotypic methods.
METHODS: In total, 110 consecutive non-repetitive isolates of K. pneumoniae were analyzed. Minimal inhibitory concentrations (MICs) of imipenem were determined. The mechanism of resistance was evaluated by imipenem-EDTA combined disk test and modified Hodge test. PCR method was used for the detection of blaVIM, blaIMP, blaNDM-1, blaOXA-48 and KPC genes.
RESULTS: Totally, 8 (7.3 %) isolates were resistant to imipenem, showing MIC ≥4 µg/mL. Based on imipenem-EDTA combined disk test, all imipenem-resistant isolates were metallo-beta-lactamase (MBL) positive. PCR confirmed that 6 (75%) isolates were blaNDM-1 positive. Other resistance genes (blaVIM, blaIMP, blaOXA-48 and KPC) were not detected.
CONCLUSIONS: Based on this study, the prevalence of CRKP strains was not at a high level, however, continuous monitoring of antibiotic resistance should be performed to control dissemination of CRKP infections.
METHODS: In total, 110 consecutive non-repetitive isolates of K. pneumoniae were analyzed. Minimal inhibitory concentrations (MICs) of imipenem were determined. The mechanism of resistance was evaluated by imipenem-EDTA combined disk test and modified Hodge test. PCR method was used for the detection of blaVIM, blaIMP, blaNDM-1, blaOXA-48 and KPC genes.
RESULTS: Totally, 8 (7.3 %) isolates were resistant to imipenem, showing MIC ≥4 µg/mL. Based on imipenem-EDTA combined disk test, all imipenem-resistant isolates were metallo-beta-lactamase (MBL) positive. PCR confirmed that 6 (75%) isolates were blaNDM-1 positive. Other resistance genes (blaVIM, blaIMP, blaOXA-48 and KPC) were not detected.
CONCLUSIONS: Based on this study, the prevalence of CRKP strains was not at a high level, however, continuous monitoring of antibiotic resistance should be performed to control dissemination of CRKP infections.