MedElevator Войти в исследования
← Поиск публикаций

DOI 10.1016/j.jcv.2026.105940

Медицинская библиотека · Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2026

Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN).

Benschop Kimberley S M, Zwagemaker Florian, Andersson-Li Lili, Andrés Cristina, Antón Andrés, Berengua Carla, Berginc Natasa, Bessaud Maël, Bisseux Maxime, Bujaki Erika, Canning Benjamin, Christiansen Claus Bohn, Couderé Karen, Broberg Eeva K, Cassidy Hayley, Castilletti Concetta, Celma Cristina, Cinek Ondrej, Deézsi-Magyar Nóra, Eis-Hübinger Anna M, Flipse Jacky, Jiřincová Helena, Gatej Raluca, Georgieva Irina, Giardina Federica, González-Sánchez Alejandra, Hack Berkin, Helfferich Jelte, Hutchings Stephanie, Hietanen Eero, Hönemann Mario, Virant Monika Jevšnik, Kalogera Eleni, Johannesen Caroline Klint, Kenicer Juliet, Kleines Michael, Lagarejos Eduardo, Landaas Elisabeth Toverud, Kandulu Chikondi C, Xavier López Labrador F, Lunar Maja M, Maier Melanie, Majumdar Manasi, Martin Javier, McClure C Patrick, Muñoz-Almagro Carmen, Ošep Anja, Øverbø Joakim, Palminha Paula, Papa Anna, Pariani Elena, Pellegrinelli Laura, Pietsch Corinna, Piralla Antonio, Poljak Mario, Pomari Elena, Prats-Méndez Ignasi, Rector Annabel, Reuter Gábor, Riess Maximilian, Ruta Simona, Schibler Manuel, Nilsen Hans-Johnny Schjelderup, Simmonds Peter, Sourvinos George, Szomor Katalin, Susi Petri, Tabain Irena, Vallely Pamela, von Eije Karin J, Weil Merav, Wieczorek Magdalena, Wollants Elke, Wolthers Katja C, Zuckerman Neta S, Fischer Thea K, Harvala Heli

Consensus StatementСтатьяПеревод в очереди

Аннотация

Enteroviruses (EVs) are a common cause of a wide spectrum of infectious diseases, ranging from mild respiratory illnesses to severe neurological conditions, particularly affecting children. Current molecular methods, such as 5'UTR-based PCR for detection and (partial) VP1 gene sequencing for typing, are widely utilized. However, Next-Generation Sequencing (NGS), and bioinformatics offer a comprehensive alternative, enabling full-genome analyses for improved virus characterization, genomic epidemiological surveillance, and outbreak investigation. Despite its advantages, implementation of NGS poses challenges, particularly in standardizing and optimizing laboratory workflows (wet-lab) and bioinformatics analyses (dry-lab), methods that are not often readily accessible in many laboratories. Here, we discuss the potential of NGS as a tool for EV detection/characterization in clinical virology, public health, and research settings. We provide practical options for actions for implementing NGS to advance the understanding and management of enterovirus infections. These recommendations are based on expert discussions during the recent European non-polio enterovirus network (ENPEN) workshop held in Corfu, Greece, on 23-24 May 2024, aiming to guide harmonization of NGS practices across clinical, public health, and research settings.

Для цитирования: Benschop Kimberley S M, Zwagemaker Florian, Andersson-Li Lili, Andrés Cristina, Antón Andrés, Berengua Carla, Berginc Natasa, Bessaud Maël, Bisseux Maxime, Bujaki Erika, Canning Benjamin, Christiansen Claus Bohn, Couderé Karen, Broberg Eeva K, Cassidy Hayley, Castilletti Concetta, Celma Cristina, Cinek Ondrej, Deézsi-Magyar Nóra, Eis-Hübinger Anna M, Flipse Jacky, Jiřincová Helena, Gatej Raluca, Georgieva Irina, Giardina Federica, González-Sánchez Alejandra, Hack Berkin, Helfferich Jelte, Hutchings Stephanie, Hietanen Eero, Hönemann Mario, Virant Monika Jevšnik, Kalogera Eleni, Johannesen Caroline Klint, Kenicer Juliet, Kleines Michael, Lagarejos Eduardo, Landaas Elisabeth Toverud, Kandulu Chikondi C, Xavier López Labrador F, Lunar Maja M, Maier Melanie, Majumdar Manasi, Martin Javier, McClure C Patrick, Muñoz-Almagro Carmen, Ošep Anja, Øverbø Joakim, Palminha Paula, Papa Anna, Pariani Elena, Pellegrinelli Laura, Pietsch Corinna, Piralla Antonio, Poljak Mario, Pomari Elena, Prats-Méndez Ignasi, Rector Annabel, Reuter Gábor, Riess Maximilian, Ruta Simona, Schibler Manuel, Nilsen Hans-Johnny Schjelderup, Simmonds Peter, Sourvinos George, Szomor Katalin, Susi Petri, Tabain Irena, Vallely Pamela, von Eije Karin J, Weil Merav, Wieczorek Magdalena, Wollants Elke, Wolthers Katja C, Zuckerman Neta S, Fischer Thea K, Harvala Heli. Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN).. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 2026. DOI: 10.1016/j.jcv.2026.105940.

Предметные рубрики MeSH

HumansComputational BiologyEnterovirusEnterovirus InfectionsEuropeGenome, ViralHigh-Throughput Nucleotide SequencingPublic Health
Подготовка и перевод публикации
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…

История · было → стало

Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.

Тип публикации указан по данным источника. Уровень доказательности автоматически не присваивается.