Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2008
A new model of end-to-side nerve graft for multiple branch reconstruction: end-to-side cross-face nerve graft in rats.
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Matsuda Ken, Kakibuchi Masao, Kubo Tateki, Tomita Koichi, Fujiwara Toshihiro, Hattori Ryo, Yano Kenji, Hosokawa Ko
Аннотация
BACKGROUND: The effectiveness of end-to-side nerve graft for multiple branch reconstruction was confirmed using a new rat four-branch facial nerve reconstruction model with end-to-side cross-face nerve graft.
METHODS: Forty Lewis rats were randomly divided into four groups with different reconstruction methods for four branches (bilateral buccal and marginal branches) of the bilateral facial nerves as follows: group I (n=12), single sciatic nerve graft with end-to-side neurorrhaphy; group II (n=12), four cable grafts using two sciatic and two ulnar nerves with end-to-end neurorrhaphy; group III (n=8), no repair; and group IV (n=8), sham operation. The four groups were compared with double retrograde tracing of the facial nucleus, electrophysiological and histomorphometrical assessment of the reconstructed facial nerve.
RESULTS: Although there were no significant differences between groups I and II in the electrophysiological tests, group I showed more uniform and better reinnervation in the histomorphometrical assessment. Retrograde tracing of facial nucleus revealed significantly higher number of double-labeled neurons in group I although the total number of labeled neurons was not different between the two groups.
CONCLUSIONS: End-to-side nerve graft shows a good functional recovery, requires less graft, and is easy to perform. With the availability of the side of the nerve graft itself as a nerve coaptation site, it can be an effective alternative in facial nerve reconstruction and be of great value in various kinds of peripheral nerve surgery.
METHODS: Forty Lewis rats were randomly divided into four groups with different reconstruction methods for four branches (bilateral buccal and marginal branches) of the bilateral facial nerves as follows: group I (n=12), single sciatic nerve graft with end-to-side neurorrhaphy; group II (n=12), four cable grafts using two sciatic and two ulnar nerves with end-to-end neurorrhaphy; group III (n=8), no repair; and group IV (n=8), sham operation. The four groups were compared with double retrograde tracing of the facial nucleus, electrophysiological and histomorphometrical assessment of the reconstructed facial nerve.
RESULTS: Although there were no significant differences between groups I and II in the electrophysiological tests, group I showed more uniform and better reinnervation in the histomorphometrical assessment. Retrograde tracing of facial nucleus revealed significantly higher number of double-labeled neurons in group I although the total number of labeled neurons was not different between the two groups.
CONCLUSIONS: End-to-side nerve graft shows a good functional recovery, requires less graft, and is easy to perform. With the availability of the side of the nerve graft itself as a nerve coaptation site, it can be an effective alternative in facial nerve reconstruction and be of great value in various kinds of peripheral nerve surgery.