Emerging infectious diseases · 2015
Isolation of Onchocerca lupi in Dogs and Black Flies, California, USA.
СтатьяResearch Support, N.I.H., IntramuralНазвание и аннотация: Перевод пока не подготовлен
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Hassan Hassan K, Bolcen Shanna, Kubofcik Joseph, Nutman Thomas B, Eberhard Mark L, Middleton Kelly, Wekesa Joseph Wakoli, Ruedas Gimena, Nelson Kimberly J, Dubielzig Richard, De Lombaert Melissa, Silverman Bruce, Schorling Jamie J, Adler Peter H, Unnasch Thomas R, Beeler Emily S
Аннотация
In southern California, ocular infections caused by Onchocerca lupi were diagnosed in 3 dogs (1 in 2006, 2 in 2012). The infectious agent was confirmed through morphologic analysis of fixed parasites in tissues and by PCR and sequencing of amplicons derived from 2 mitochondrially encoded genes and 1 nuclear-encoded gene. A nested PCR based on the sequence of the cytochrome oxidase subunit 1 gene of the parasite was developed and used to screen Simulium black flies collected from southern California for O. lupi DNA. Six (2.8%; 95% CI 0.6%-5.0%) of 213 black flies contained O. lupi DNA. Partial mitochondrial16S rRNA gene sequences from the infected flies matched sequences derived from black fly larvae cytotaxonomically identified as Simulium tribulatum. These data implicate S. tribulatum flies as a putative vector for O. lupi in southern California.