Cryo letters · 2020
Treatment with Melatonin Enhances the Embryo Quality and the Development of Vitrified/warmed Eight Cell Mouse Embryos by Solid Surface Vitrification (SSV).
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Caglar K, Kocabay A, Taskin A C, Arat S
Аннотация
BACKGROUND: Melatonin is an endocrine hormone secreted from the pineal gland located outside the blood-brain barrier.
OBJECTIVE: In this study, in vitro propagated eight-cell mouse embryos were vitrified by the Solid Surface Vitrification (SSV) method and after thawing, their in vitro development and embryo qualities in melatonin added media were investigated.
METHODS: Pronuclear stage embryos obtained from super ovulated B6CBAF1/J strain mice, were cultured until the eight-cell stage. Then these eight-cell embryos were vitrified by the SSV method and after thawing, cultured in melatonin added media at 37°C and 5 %CO2 conditions until the blastocyst stage.
RESULT: In the experimental period, in vitro embryo development rates of the control, SSV and +10-12 M melatonin groups were observed as 97%, 86% and 93%, respectively.
CONCLUSION: Our results indicated that melatonin addition slightly increased the development rates and total cell numbers of embryos vitrified by the SSV method.
OBJECTIVE: In this study, in vitro propagated eight-cell mouse embryos were vitrified by the Solid Surface Vitrification (SSV) method and after thawing, their in vitro development and embryo qualities in melatonin added media were investigated.
METHODS: Pronuclear stage embryos obtained from super ovulated B6CBAF1/J strain mice, were cultured until the eight-cell stage. Then these eight-cell embryos were vitrified by the SSV method and after thawing, cultured in melatonin added media at 37°C and 5 %CO2 conditions until the blastocyst stage.
RESULT: In the experimental period, in vitro embryo development rates of the control, SSV and +10-12 M melatonin groups were observed as 97%, 86% and 93%, respectively.
CONCLUSION: Our results indicated that melatonin addition slightly increased the development rates and total cell numbers of embryos vitrified by the SSV method.