[Detection of anti-Trichinella spiralis antibody by indirect ELISA using recombinant protein as antigen].

OBJECTIVE

To study the specificity, sensitivity of diagnostic antigen for Trichinella spiralis (T.s).

METHODS

T668 recombinant protein from highly efficient expression of newborn larvae stage-specific gene of T.s in E. coli as diagnostic antigen. By using negative and positive sera from rabbit, pig, human as the first antibody, and goat-anti-rabbit IgG, goat-anti-pig IgG, goat-anti-human IgG labeled with HRP as the secondary antibody, indirect ELISA method was established for detecting anti-T.s antibody, while excretory-secretory (ES) antigen of T.s muscle larvae was used as control.

RESULTS

Sera from rabbit, pig and human were detected by T668 recombinant protein as antigen, which showed a positive rate of 100% with 0.016 microg/well. There was no difference on the results between the T668 recombinant antigen and the ES antigen for diagnosing T.s-infection.

CONCLUSION

The T668 recombinant antigen is promising in substituting ES antigen in the detection of anti-T.s antibody.