Anaerobe · 2025
Comparison of disc diffusion and Sensititre® Broth Microdilution methods for antimicrobial susceptibility testing of Bacteroides fragilis group clinical isolates following the French guidelines.
СтатьяСравнительное исследованиеНазвание и аннотация: Перевод в очереди
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Delvallez Gauthier, Diancourt Laure, Germond Julie, Campagne Pascal, Dupuy Bruno
Аннотация
OBJECTIVES: This study aims to evaluate the concordance between the disc diffusion test (DDT) and custom-designed Sensititre® Broth Microdilution plates and to estimate their respective sensitivity and specificity for antimicrobial susceptibility testing (AST) of Bacteroides fragilis group (BFG) clinical isolates.
METHODS: AST was performed on 126 BFG isolates using both DDT and Sensititre® methods according to the 2024 CA-SFM guidelines. Cohen's kappa coefficients were used to assess concordance, while a Bayesian approach estimated sensitivity and specificity with 95 % confidence intervals (95 % CIs) for amoxicillin-clavulanate (AMC), piperacillin-tazobactam (PTZ), imipenem (IPM), clindamycin (CLI), metronidazole (MTR), and moxifloxacin (MXF).
RESULTS: Cohen's kappa coefficients with 95 % CIs for AMC, PTZ, IPM, CLI, MTR, and MXF were 0.92 (0.85-1.00), 0.70 (0.56-0.83), 0.96 (0.92-1.00), 0.98 (0.94-1.00), 0.96 (0.89-1.00), and 0.70 (0.58-0.82), respectively. DDT demonstrated sensitivity > 0.8 for all antibiotics, and specificity > 0.8 except for PTZ (0.51; 0.37-0.65) and CLI (0.71; 0.58-0.82). Sensititre® exhibited sensitivity > 0.9 for all antibiotics except for PTZ (0.86; 0.70-0.99), and specificity > 0.9 except for MXF (0.88; 0.75-0.99).
CONCLUSIONS: DDT and Sensititre® Broth Microdilution demonstrated strong concordance for most antibiotics. Predictive values estimated using Bayesian model showed that Sensititre® offered the highest overall sensitivity and specificity.
METHODS: AST was performed on 126 BFG isolates using both DDT and Sensititre® methods according to the 2024 CA-SFM guidelines. Cohen's kappa coefficients were used to assess concordance, while a Bayesian approach estimated sensitivity and specificity with 95 % confidence intervals (95 % CIs) for amoxicillin-clavulanate (AMC), piperacillin-tazobactam (PTZ), imipenem (IPM), clindamycin (CLI), metronidazole (MTR), and moxifloxacin (MXF).
RESULTS: Cohen's kappa coefficients with 95 % CIs for AMC, PTZ, IPM, CLI, MTR, and MXF were 0.92 (0.85-1.00), 0.70 (0.56-0.83), 0.96 (0.92-1.00), 0.98 (0.94-1.00), 0.96 (0.89-1.00), and 0.70 (0.58-0.82), respectively. DDT demonstrated sensitivity > 0.8 for all antibiotics, and specificity > 0.8 except for PTZ (0.51; 0.37-0.65) and CLI (0.71; 0.58-0.82). Sensititre® exhibited sensitivity > 0.9 for all antibiotics except for PTZ (0.86; 0.70-0.99), and specificity > 0.9 except for MXF (0.88; 0.75-0.99).
CONCLUSIONS: DDT and Sensititre® Broth Microdilution demonstrated strong concordance for most antibiotics. Predictive values estimated using Bayesian model showed that Sensititre® offered the highest overall sensitivity and specificity.