Protocatechuic acid, a new active substance against the challenge of avian infectious bursal disease virus.

The present study aims to investigate the potential antiviral activity of protocatechuic acid (PCA) and its mechanism against infectious bursal disease virus (IBDV) infection. In the curative test, dosages of PCA of 40, 20, and 10 mg/kg, the survival rate was 90, 90, and 60%, respectively, and the BW gain was 36.63, 31.85, and 51.8%, respectively. The survival rate for the Astragalus polysaccharide (ASP) group was significantly lower than those of the birds treated with 20 mg/kg or 40 mg/kg of PCA. The bursa indeces of chickens in 40 mg/kg, 20 mg/kg, and ASP groups were significantly higher than that of the infection group, whereas a significant increase of the spleen index was found in birds with 20 mg/kg PCA in comparison with other challenged groups. The birds treated with 20 mg/kg or 40 mg/kg of 3,4-dihydroxybenzoic acid also showed slightly higher levels of IBDV clearance in the bursa of Fabricius. Furthermore, the chickens treated with 20 mg/kg of PCA induced a significant lymphocyte proliferation and a significant increase in the CD4+/CD8+ ratio in comparison with the ASP chickens. These results imply that chickens treated with 20 mg/kg of PCA for 5 d could effectively induce active nonspecific immune responses against the IBDV infection.