Establishment of B4-100 and B4-C4, clonal canine multiple myeloma cell lines and their application in generating monoclonal antibody-producing fully canine hybridomas.

We report on the establishment of the unique canine multiple myeloma cloned cell lines B4-100 and B4-C4, established from the peripheral blood of a canine patient with suspected lymphoma. The cloned cells were analyzed for morphologic traits, proliferation rates, cell doubling times, as well as canine immunoglobulin production by flow cytometry. The cells were found to express IgE in the cell lysate by western blotting but did not express HGPRT, and were unable to grow in hypoxanthine-aminopterin-thymidine medium. When the cells were fused with canine peripheral blood mononuclear cells in vitro, hybridomas producing canine immunoglobulins in the culture supernatant could be generated. To our knowledge, this is the first report on establishment of canine myeloma cell lines and we submit that these cell lines may provide opportunities for the production of fully caninized antibodies with potential for therapeutic applications.